工业微生物
工業微生物
공업미생물
INDUSTRIAL MICROBIOLOGY
2015年
1期
1-6
,共6页
郭香%张硕%唐敬玉%邹小周%唐晓燕%陈琳%洪枫
郭香%張碩%唐敬玉%鄒小週%唐曉燕%陳琳%洪楓
곽향%장석%당경옥%추소주%당효연%진림%홍풍
细菌纤维素%纸浆废料%酶水解%木醋杆菌
細菌纖維素%紙漿廢料%酶水解%木醋桿菌
세균섬유소%지장폐료%매수해%목작간균
bacterial cellulose%waste fiber sludge%enzymatic hydrolysis%Gluconacetobacter xylinus
细菌纤维素(bacterial cellulose,BC)是一种由微生物产生的具有纳米结构的纤维素材料。BC生产的培养基成本偏高,限制了其规模化工业生产和商业应用。为开发新的BC生产原料,通过Cellic CTec 2纤维素酶直接水解硫酸盐和亚硫酸盐两种纸浆废料获得可发酵糖,以其成功制备出BC并研究比较了两种酶解液对BC产量和结构的差异。结果表明,硫酸盐纸浆废料获得的BC产量最高,达9.0 g/L,比亚硫酸盐纸浆废料的7.7 g/L高了17%。两种原料制备的BC膜的结晶度分别为61%和66%,比葡萄糖制备的(78%)低。红外光谱分析表明,不同碳源制备的BC膜的成分没有明显差异。
細菌纖維素(bacterial cellulose,BC)是一種由微生物產生的具有納米結構的纖維素材料。BC生產的培養基成本偏高,限製瞭其規模化工業生產和商業應用。為開髮新的BC生產原料,通過Cellic CTec 2纖維素酶直接水解硫痠鹽和亞硫痠鹽兩種紙漿廢料穫得可髮酵糖,以其成功製備齣BC併研究比較瞭兩種酶解液對BC產量和結構的差異。結果錶明,硫痠鹽紙漿廢料穫得的BC產量最高,達9.0 g/L,比亞硫痠鹽紙漿廢料的7.7 g/L高瞭17%。兩種原料製備的BC膜的結晶度分彆為61%和66%,比葡萄糖製備的(78%)低。紅外光譜分析錶明,不同碳源製備的BC膜的成分沒有明顯差異。
세균섬유소(bacterial cellulose,BC)시일충유미생물산생적구유납미결구적섬유소재료。BC생산적배양기성본편고,한제료기규모화공업생산화상업응용。위개발신적BC생산원료,통과Cellic CTec 2섬유소매직접수해류산염화아류산염량충지장폐료획득가발효당,이기성공제비출BC병연구비교료량충매해액대BC산량화결구적차이。결과표명,류산염지장폐료획득적BC산량최고,체9.0 g/L,비아류산염지장폐료적7.7 g/L고료17%。량충원료제비적BC막적결정도분별위61%화66%,비포도당제비적(78%)저。홍외광보분석표명,불동탄원제비적BC막적성분몰유명현차이。
Bacterial cellulose (BC)is a nanostructured polymer product of some bacteria,principally acetic acid bacteria. Compared to plant cellulose,BC has a great potential in wide applications due to its unique properties. Because of the high cost of culture medium,large-scale industrial production and commercial application of BC were limited. In this work,two different waste fiber sludges,sulfate fiber sludge (SAFS)and sulfite fiber sludge (SIFS)were easily hydro-lyzed by cellulase without prior thermochemical pretreatment and thereafter were used to produce BC successfully. The yield and structure of BC produced from the two different fiber sludges were compared. The results indicated that the yield of BC from SAFS were 9. 0 g/L,which was about 17% higher than that of SIFS (7. 7 g/L). The crystallinity of the BC membranes differed due to the culture media. The crystallinity of BC from SAFS hydrolysate was around 61%,while that from SIFS hydrolysate was around 66%,both of them were lower than that (78%)from the glucose-based reference medi-um. ATR-FTIR analysis showed that there were no significant differences in the chemical compositions of BC membranes obtained from the two waste fiber sludges and glucose-based media.