CAJ | 학술논문

目的：探讨海南萝芙木提取物对葡聚糖硫酸钠（DSS）小鼠结肠炎的作用及机制。方法80只BALB/c小鼠随机分为：（1）正常对照组；（2）模型组；（3）模型＋柳氮磺胺嘧啶治疗组；（4）模型＋海南萝芙木提取物治疗组。实验结束后剖取各组小鼠结肠炎症组织，HE染色，组织学评分；免疫组化方法检测各组小鼠肠黏膜组织中NF-κB抑制蛋白（IκB-α）阳性细胞表达率， ELISA方法检测定小鼠结肠组织白细胞介素-4（IL-4）、白细胞介素-13（IL-13）表达。结果柳氮磺胺嘧啶治疗组和海南萝芙木提取物治疗组小鼠一般情况好于DSS模型组，DAI积分低于DSS模型组；结肠组织显微镜检查，动物结肠炎症程度低于DSS模型组；动物结肠黏膜细胞中IκB-α阳性细胞表达率显著高于DSS模型组；小鼠结肠组织中IL-4、IL-13水平均显著高于DSS模型组。结论海南萝芙木提取物果胶多糖可能通过于预 IκB-α蛋白表达，进而抑制溃疡性结肠炎症细胞中NF-κB活性，抑制IL-4、IL-13等炎症因子，产生抗炎效果。
목적：탐토해남라부목제취물대포취당류산납（DSS）소서결장염적작용급궤제。방법80지BALB/c소서수궤분위：（1）정상대조조；（2）모형조；（3）모형＋류담광알밀정치료조；（4）모형＋해남라부목제취물치료조。실험결속후부취각조소서결장염증조직，HE염색，조직학평분；면역조화방법검측각조소서장점막조직중NF-κB억제단백（IκB-α）양성세포표체솔， ELISA방법검측정소서결장조직백세포개소-4（IL-4）、백세포개소-13（IL-13）표체。결과류담광알밀정치료조화해남라부목제취물치료조소서일반정황호우DSS모형조，DAI적분저우DSS모형조；결장조직현미경검사，동물결장염증정도저우DSS모형조；동물결장점막세포중IκB-α양성세포표체솔현저고우DSS모형조；소서결장조직중IL-4、IL-13수평균현저고우DSS모형조。결론해남라부목제취물과효다당가능통과우예 IκB-α단백표체，진이억제궤양성결장염증세포중NF-κB활성，억제IL-4、IL-13등염증인자，산생항염효과。
Objective To investigate the effects and explore the regulating mechanism of extractive from Rauvolfiaverticillata (Lour.) Baill. var. hainanensis Tsiang in the mechanism of DSS induced Mice Colitis. Methods 80 BALB/c mice were randomly divided into 4 groups:(1)normal control group;(2)model group;(3)model plus salicylazosulfa-pyridine (SASP, used as a positive control drug) (n=20/group);(4)model plus extractive from Rauvolfiaverticillata (Lour.) Baill. var. hainanensis Tsiang. After the end of the experiment, each murine colitis tissue section was taken, HE staining;histopathological damage and histological scores were observed; With immunohistochemical staining IκB-α expression in colon tissues were detected; With enzyme-linked immunosorbent assay (ELISA) interleukin-4, interleukin-13 levels in colon tissue were measured. Results Compared with the model plus pecticpolysaccharides group and model plus SASP group, the DAI value was significantly greater in mice treated with DSS (P<0.05). Compared with model group, the colitis symptoms were relieved and IκB-αprotein was increased in mice of the model plus pecticpolysaccharides group and model plus SASP group (P<0.05). The production of IL-4 and IL-13 were increased in mice of the model plus pecticpolysaccharides group and model plus SASP group (P<0.05). Conclusion Pecticpolysaccharides extracted from Rauvolfia verticillata (Lour. )Baill. var. hainanensis Tsiang ameliorated ulcerative colitis and might exhibit its anti-inflammatory effects via increased expression of IκB-α proteins and suppressing NF-κB translocation and interleukin-4 and interleukin-13 levels.