CAJ | 학술논문

目的：优化五味子抗氧化物的提取条件；分析湖北产和辽宁产五味子提取物的 DPPH清除能力。方法以 DPPH自由基的清除能力为参考指标，采用单因素实验的方法，考察溶剂种类、乙醇浓度、料液比、提取时间、提取次数和提取方法等因素对五味子提取物抗氧化作用的影响。结果五味子抗氧化物的最优提取条件为：以60％的乙醇为提取溶剂，超声法提取，料液比为1∶30（ g? mL －1），提取次数3次，每次提取20min，室温提取；湖北产和辽宁产五味子提取物清除DPPH自由基的IC50分别为3．79mg? mL －1和7．60mg? mL －1。结论五味子提取物具有较强的抗氧化作用，且湖北产五味子提取物的抗氧化作用比辽宁产五味子的强。
목적：우화오미자항양화물적제취조건；분석호북산화료녕산오미자제취물적 DPPH청제능력。방법이 DPPH자유기적청제능력위삼고지표，채용단인소실험적방법，고찰용제충류、을순농도、료액비、제취시간、제취차수화제취방법등인소대오미자제취물항양화작용적영향。결과오미자항양화물적최우제취조건위：이60％적을순위제취용제，초성법제취，료액비위1∶30（ g? mL －1），제취차수3차，매차제취20min，실온제취；호북산화료녕산오미자제취물청제DPPH자유기적IC50분별위3．79mg? mL －1화7．60mg? mL －1。결론오미자제취물구유교강적항양화작용，차호북산오미자제취물적항양화작용비료녕산오미자적강。
ABSTRACT:OBJECTIVE To optimize the extraction conditions of Schisandra antioxidant and to analyze the DP-PH scavenging ability of Hubei and Liaoning production of Schisandra chinensis extracts .METHODS Based on DPPH free radical scavenging activity of extracts of Schisandra chinensis and using single factor experiment ,extrac-ting conditions were optimized through a series of comparative researches on the influences of solvent types ,ethanol concentration ,solid-liquid ratio ,extraction method ,extraction time and extraction frequencies .RESULTS The opti-mal conditions for extracting Schisandra chinensis were as follows:using 60%EtOH as extracting solvent ,ultrasonic assisted extraction method,solid-liquid ratio(m/V) was 1g:30mL,extraction time 20min,the sample was extracted 3 times and each time for 20minutes,extraction temperature was room temperature.Under this condition above-men-tioned,the DPPH free radical scavenging activity of Schisandra chinensis from Hubei and Liaoning production were detected,the IC50 of which were 3.79mg? mL-1 and 7.60mg? mL-1 respectively.CONCLUSION The results a-chieved show Schisandra extract has strong antioxidation and the DPPH antioxidant activity of Hubei sample is stron -ger than that of Liaoning one .