CAJ | 학술논문

目的：探讨CADM1在骨肉瘤细胞中的表达及其与MMP-2、MMP-9的关系。方法：用Western Blot法检测hFOB 1.19人SV40转染成骨细胞及骨肉瘤细胞Saos-2、U-2os、MG-63中CADM1、MMP-2、MMP-9的表达情况，使用图象分析软件Image—Pro Plus 5．0对以上四种细胞中三种蛋白的表达情况进行半定量测定，并运用统计学方法检测在骨肉瘤细胞中CADM1的表达情况及其与MMP-2、MMP-9的关系。结果：与hFOB 1.19人SV40转染成骨细胞相比，骨肉瘤细胞Saos-2、U-2os、MG-63中CADM1的表达水平明显降低、MMP-2的表达水平明显升高。骨肉瘤细胞Saos-2、MG-63中MMP-9的表达呈缺失状态。CADM1与MMP-2的表达具有负相关性，与MMP-9的表达不具有相关性。结论：CADM1在骨肉瘤细胞中的表达呈明显降低的表现，其可能与MMP-2、MMP-9在骨肉瘤的发病机制中发挥着重要的作用。
목적：탐토CADM1재골육류세포중적표체급기여MMP-2、MMP-9적관계。방법：용Western Blot법검측hFOB 1.19인SV40전염성골세포급골육류세포Saos-2、U-2os、MG-63중CADM1、MMP-2、MMP-9적표체정황，사용도상분석연건Image—Pro Plus 5．0대이상사충세포중삼충단백적표체정황진행반정량측정，병운용통계학방법검측재골육류세포중CADM1적표체정황급기여MMP-2、MMP-9적관계。결과：여hFOB 1.19인SV40전염성골세포상비，골육류세포Saos-2、U-2os、MG-63중CADM1적표체수평명현강저、MMP-2적표체수평명현승고。골육류세포Saos-2、MG-63중MMP-9적표체정결실상태。CADM1여MMP-2적표체구유부상관성，여MMP-9적표체불구유상관성。결론：CADM1재골육류세포중적표체정명현강저적표현，기가능여MMP-2、MMP-9재골육류적발병궤제중발휘착중요적작용。
[ABSTRACT]AIM:To investigate the expression of CADM1 in osteosarcoma cells , and its relationship with MMP-2 and MMP-9.METHODS: The protein levels of CADM1 ,MMP-2 and MMP-9 in 1 osteoblast and 3 human osteosarcoma cell lines are detected by Western blotting. The 3 kinds protein levels in 4 cell lines are measured by Image—Pro Plus 5.0 . Statistical methods is used to investigate the relationship between CADM1 and MMP-2. RESULTS: In Osteosarcoma cell lines Saos-2,U-2os and MG-63,the protein level of CADM1 is Significantly lower than hFOB 1.19 osteoblasts, and the level of MMP-2 is higher In contrast.the expression of MMP-9 is missing in Saos-2 and MG-63.In addition,the expression of CADM1 and MMP-2 are negative correlation , and the expression of CADM1 and MMP-9 are zero correlation . CONCLUSION: The expression of CADM1 in osteosarcoma cells is significantly reduced . CADM1 may play important roles in the development of osteosarcoma with MMP-2 and MMP-9 together.