CAJ | 학술논문

目的观察轴突导向因子Netrin-1在先天性巨结肠(Hirschsprung's disease,HD)患儿中正常段与痉挛段结肠中的表达情况,探讨其在HD发病机制中的作用.方法对36例HD患儿的正常段肠管(对照组)与痉挛段肠管(病变组),用免疫组织化学方法检测Netrin-1在两组中的表达.结果在对照组(正常段)肠管中肌层(纵肌、环肌)、肌间神经节及黏膜层均见Netrin-1表达,病变组(痉挛段)中肌层及黏膜层亦可见Netrin-1表达,两组间Netrin-1的表达强度差异无统计学意义(P＞0.05).结论 Netrin-1在HD患儿正常段和痉挛段肠管的肌层、黏膜层表达无显著差异,可能说明Netrin-1与HD的发生无明显相关性.
목적 관찰축돌도향인자Netrin-1재선천성거결장(Hirschsprung's disease,HD)환인중정상단여경련단결장중적표체정황,탐토기재HD발병궤제중적작용.방법 대36례HD환인적정상단장관(대조조)여경련단장관(병변조),용면역조직화학방법검측Netrin-1재량조중적표체.결과 재대조조(정상단)장관중기층(종기、배기)、기간신경절급점막층균견Netrin-1표체,병변조(경련단)중기층급점막층역가견Netrin-1표체,량조간Netrin-1적표체강도차이무통계학의의(P＞0.05).결론 Netrin-1재HD환인정상단화경련단장관적기층、점막층표체무현저차이,가능설명Netrin-1여HD적발생무명현상관성.
Objective To observe the expression and the distribution of Netrin-1 in the spastic and normal segment of colon in patients with Hirschsprungs disease (HD) and investigate the relationship between Netrin-1 and development of HD.Methods Colon specimens of 36 cases with HD were selected for this study.Normal segment of colon served as the control group,while the spastic segments of colon as the lesion group.Immunohistochemical staining was applied to detecte the expression and the distribution of Netrin-1.Results Immunohistochemical staining showed that the expression of netrin-1 was positive in the intestinal muscular layers (longitudinal muscle,circular muscles),myenteric ganglias and mucosal layers in the control group.However,the expression of Netrin-1 was also positive in muscular layers and mucosal layer in the lesion group.There was no significant difference in the expression intensity of Netrin-1 between the two groups (P ＞0.05).Conclusion The expression of Netrin-1 in both the control group and the lesion group may indicate that netrin-1 have no significant correlation with genesis of HD.