医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2015年
8期
345-347
,共3页
哮喘%中性粒细胞%气道炎症%地塞米松
哮喘%中性粒細胞%氣道炎癥%地塞米鬆
효천%중성립세포%기도염증%지새미송
Asthma%Neutrophil%Airway inflammation%Dexamethasone
目的：观察地塞米松对中性粒细胞性哮喘（neutrophilic asthma，NA）模型小鼠气道炎症的影响。方法：C57BL雌性小鼠随机分成NA组、NA地塞米松干预（neutrophilic asthma treating with dexamethasone，NAD）组和正常（NC）组，每组8只。NA、NAD组予卵蛋白、脂多糖致敏并卵蛋白激发；NAD组激发前腹腔注射地塞米松；NC组不做处理。小鼠肺功能仪检测气道阻力，以阻力倍增值评价气道反应性；检测支气管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）有核细胞浓度及分类比例；流式细胞术检测脾脏Th17细胞及ki-67+Th17细胞比例；ELISA检测BALF IL-17浓度。结果（1）NA组气道阻力倍增值显著高于NC组（P<0.05）。（2） NAD组BALF有核细胞浓度、NEU%、EOS%均显著低于NA组，但仍高于NC组（均P<0.05）。（3）NAD组脾脏Th17细胞比例显著低于NA组，但仍高于NC组（均P<0.05）；NA、NAD组脾脏ki-67+Th17细胞比例均显著高于NC组（均P<0.05），但NA、NAD组间差异无统计学意义。（4）NAD组BALF IL-17浓度显著低于NA组，但仍高于NC组（均P<0.05）。结论：地塞米松可能通过抑制Th17细胞及IL-17表达，减轻NA模型小鼠气道炎症；Th17细胞增殖功能增强且不受地塞米松影响可能与气道炎症持续存在有关。
目的：觀察地塞米鬆對中性粒細胞性哮喘（neutrophilic asthma，NA）模型小鼠氣道炎癥的影響。方法：C57BL雌性小鼠隨機分成NA組、NA地塞米鬆榦預（neutrophilic asthma treating with dexamethasone，NAD）組和正常（NC）組，每組8隻。NA、NAD組予卵蛋白、脂多糖緻敏併卵蛋白激髮；NAD組激髮前腹腔註射地塞米鬆；NC組不做處理。小鼠肺功能儀檢測氣道阻力，以阻力倍增值評價氣道反應性；檢測支氣管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）有覈細胞濃度及分類比例；流式細胞術檢測脾髒Th17細胞及ki-67+Th17細胞比例；ELISA檢測BALF IL-17濃度。結果（1）NA組氣道阻力倍增值顯著高于NC組（P<0.05）。（2） NAD組BALF有覈細胞濃度、NEU%、EOS%均顯著低于NA組，但仍高于NC組（均P<0.05）。（3）NAD組脾髒Th17細胞比例顯著低于NA組，但仍高于NC組（均P<0.05）；NA、NAD組脾髒ki-67+Th17細胞比例均顯著高于NC組（均P<0.05），但NA、NAD組間差異無統計學意義。（4）NAD組BALF IL-17濃度顯著低于NA組，但仍高于NC組（均P<0.05）。結論：地塞米鬆可能通過抑製Th17細胞及IL-17錶達，減輕NA模型小鼠氣道炎癥；Th17細胞增殖功能增彊且不受地塞米鬆影響可能與氣道炎癥持續存在有關。
목적：관찰지새미송대중성립세포성효천（neutrophilic asthma，NA）모형소서기도염증적영향。방법：C57BL자성소서수궤분성NA조、NA지새미송간예（neutrophilic asthma treating with dexamethasone，NAD）조화정상（NC）조，매조8지。NA、NAD조여란단백、지다당치민병란단백격발；NAD조격발전복강주사지새미송；NC조불주처리。소서폐공능의검측기도조력，이조력배증치평개기도반응성；검측지기관폐포관세액（bronchoalveolar lavage fluid，BALF）유핵세포농도급분류비례；류식세포술검측비장Th17세포급ki-67+Th17세포비례；ELISA검측BALF IL-17농도。결과（1）NA조기도조력배증치현저고우NC조（P<0.05）。（2） NAD조BALF유핵세포농도、NEU%、EOS%균현저저우NA조，단잉고우NC조（균P<0.05）。（3）NAD조비장Th17세포비례현저저우NA조，단잉고우NC조（균P<0.05）；NA、NAD조비장ki-67+Th17세포비례균현저고우NC조（균P<0.05），단NA、NAD조간차이무통계학의의。（4）NAD조BALF IL-17농도현저저우NA조，단잉고우NC조（균P<0.05）。결론：지새미송가능통과억제Th17세포급IL-17표체，감경NA모형소서기도염증；Th17세포증식공능증강차불수지새미송영향가능여기도염증지속존재유관。
Objective To observe the effect of dexamethasone on airway inflammation in the mouse model of Neutrophilic Asthma. Methods Female C57BL mice were randomly divided into NA, NAD and NC group.NA、NAD mice were sensitized by ovalbumin OVA and LPS and challenged by OVA. NAD mice were treated with dexamethasone at beginning of each challenge.NC mice without treatment.Airway resistance were measured and fold increase were caculated as an assessment for AHR.Total white blood cell concentration and classification of proportion were determined in the BALF.Percentages of Th17 cells and Ki-67+Th17 cells in the spleen were determined by FCM.BALF IL-17 concentration were determined by ELISA. Results (1) Fold increase of airway resistance in the NA group were higher than that of the NC group (each P<0.05).(2)BALF total cell count, neutrophil and eosinophil percentages in the NAD group were lower than that of the NA group (each P<0.05), but were higher than that of the NC group(each P<0.05). (3) Th17 cell percentages in the spleen of the NAD group were lower than that of the NA group, but were higher than that of the NC group(each P<0.05). Ki-67+Th17 cells percentage in the spleen of the NA and NAD group were higher than that of the NC group(each P<0.05);Hoever,no difference were found in the NA group compared with that of the NAD group(4)BALF IL-17 concentration in the NAD group were lower than that of the NA group, but were higher than that of the NC group(each P<0.05). Conclusions Airway inflammation in the mouse model of NA were improved by dexamethasone via its inhibition on the exspression of Th17 cells.The enhanced function of Th17 cells proliferation not influenced by dexamethasone,may be responsible for the persistence of the inflammation.
