CAJ | 학술논문

本试验建立了化妆品（爽肤水和润肤乳）中禁用的含氯苯酚（2，4，6‐三氯苯酚和五氯苯酚）的超高效液相色谱－串联质谱测定方法，采用Q1多离子监测模式。1．0g爽肤水和1．0g润肤乳样品经10 m L正己烷超声辅助提取后，取正己烷层45℃氮吹至近干，甲醇定容，经液相色谱分离、质谱测定，外标法定量。2，4，6‐三氯苯酚在添加水平200μg／kg和800μg／kg时，方法的回收率范围为81．6％－94．5％，五氯苯酚在添加水平50μg／kg和200μg／kg时回收率范围为65．3％－74．3％，相对标准偏差小于6％。爽肤水中2，4，6‐三氯苯酚和五氯苯酚的检出限分别为20μg／kg和3μg／kg ，定量限分别为50μg／kg和10μg／kg ；润肤乳中2，4，6‐三氯苯酚和五氯苯酚的检出限分别为30μg／kg和5μg／kg ，定量限分别为100μg／kg和20μg／kg。该方法可用于爽肤水和润肤乳中2，4，6‐三氯苯酚和五氯苯酚的快速筛查和定量分析。
본시험건립료화장품（상부수화윤부유）중금용적함록분분（2，4，6‐삼록분분화오록분분）적초고효액상색보－천련질보측정방법，채용Q1다리자감측모식。1．0g상부수화1．0g윤부유양품경10 m L정기완초성보조제취후，취정기완층45℃담취지근간，갑순정용，경액상색보분리、질보측정，외표법정량。2，4，6‐삼록분분재첨가수평200μg／kg화800μg／kg시，방법적회수솔범위위81．6％－94．5％，오록분분재첨가수평50μg／kg화200μg／kg시회수솔범위위65．3％－74．3％，상대표준편차소우6％。상부수중2，4，6‐삼록분분화오록분분적검출한분별위20μg／kg화3μg／kg ，정량한분별위50μg／kg화10μg／kg ；윤부유중2，4，6‐삼록분분화오록분분적검출한분별위30μg／kg화5μg／kg ，정량한분별위100μg／kg화20μg／kg。해방법가용우상부수화윤부유중2，4，6‐삼록분분화오록분분적쾌속사사화정량분석。
A liquid chromatography‐tandem mass spectrometry method was built to determine 2 ,4 ,6‐trichlorophenol ,pentachlorophenol in toner and moisturizer ,Q1 multiple ions scan mode was used in the experiment .1 .0 g toner and 1 .0 g moisturizer was extracted by n‐hex‐ane with ultrasonic assisted extraction .The extracts of n‐hexane were dried by nitrogen at 45℃ ,and then dissolved by methanol .Anlytes were separated by liquid chromatography ,de‐termined by mass spectrometry and quantified with external standard method .Recoveries of 2 ,4 ,6‐trichlorophenol were in range of 81 .6% -94 .5% with spike level of 200μg/kg and 800μg/kg ;recoveries of pentachlorophenol were in range of 65 .3% -74 .3% with spike level of 50μg/kg and 200μg/kg ;relative standard deviations of method w ere low er than 6% .Limit of detection of 2 , 4 , 6‐trichlorophenol and pentachlorophenol in toner was 20μg/kg 和3μg/kg ,limit of quantitation of 2 ,4 ,6‐trichlorophenol and pentachlorophenol in toner was 50μg/kg和10μg/kg ;limit of detection of 2 ,4 ,6‐trichlorophenol and pentachlorophenol in moisturizer w as 30μg/kg和5μg/kg ,limit of quantitation of 2 ,4 ,6‐trichlorophenol and pen‐tachlorophenol in moisturizer was 100μg/kg和20μg/kg .This method could be used in rapid screening and quantitative analysis of 2 ,4 ,6‐trichlorophenol ,pentachlorophenol in toner and moisturizer .