CAJ | 학술논문

目的：：采用响应面分析法( RSM)对绞股蓝总黄酮的提取工艺进行优化。方法：以总黄酮提取率为考察指标,在单因素试验基础上选择试验因素和水平,进行Box-Behnken响应面试验设计,得到最佳操作条件及二次方程响应面模型。结果：绞股蓝总黄酮提取的最佳工艺条件为：乙醇体积分数71%,料液比1∶14,超声时间32 min,该工艺条件下,绞股蓝总黄酮提取率模型预测值为4.676%,验证试验得率为4.641%。结论：回归模型拟合度良好,该提取工艺可行、可靠。
목적：：채용향응면분석법( RSM)대교고람총황동적제취공예진행우화。방법：이총황동제취솔위고찰지표,재단인소시험기출상선택시험인소화수평,진행Box-Behnken향응면시험설계,득도최가조작조건급이차방정향응면모형。결과：교고람총황동제취적최가공예조건위：을순체적분수71%,료액비1∶14,초성시간32 min,해공예조건하,교고람총황동제취솔모형예측치위4.676%,험증시험득솔위4.641%。결론：회귀모형의합도량호,해제취공예가행、가고。
Objective:To optimize the extraction process of total flavonoids in Gynostemma pentaphyllum by the method of response surface analysis ( RSM) . Methods: Based on the choice of factors and levels according to the results of single factor test, Box-Be-hnken response surface experiment was designed with the extraction rate of total flavonoids as the index to obtaln the best operation con-ditions and two equations of the response surface model. Results: The optimal extraction conditions of total flavonoids in Gynostemma pentaphyllum were as follows:the volume fraction of ethanol was 71%, the ratio of solid to liquid was 1∶14, and the ultrasonic time was 32 min. Under the conditions, the model predictive value of extraction rate of total flavonoids in Gynostemma pentaphyllum was 4. 676%, and the extraction rate in the verification test was 4. 641%. Conclusion:The fit of the regression model is good, and the ex-traction technology is feasible and reliable.