CAJ | 학술논문

目的：分析多氯联苯（ PCBs）与高脂饮食暴露后SD大鼠尿液代谢组变化，探讨PCBs对糖、脂肪等代谢的影响及可能的毒性机制，并寻找潜在的候选生物标志物。方法雄性SD大鼠随机分为对照组、高脂组、PCBs组、PCBs与高脂联合暴露组。于染毒6周收集尿液样本，采集尿液的1 H NMR谱，采用主成分分析法（ PCA）分析数据。结果 PCA得分图显示，联合暴露组与其他3组可完全区分，表明该组动物尿液代谢特征与其他3组存在较大差异。 PCA载荷图显示，联合暴露组大鼠尿液中乳酸、葡萄糖、肌酸、2-羟异戊酸含量显著上升；柠檬酸、琥珀酸、牛磺酸、马尿酸、氧化三甲胺（ TMAO）含量显著下降。结论 PCBs与高脂联合暴露导致大鼠尿液中三羧酸循环的中间产物含量降低或与能量代谢相关的代谢物蓄积，可能与线粒体功能受损、能量代谢障碍有关。这些效应可能会进一步导致糖类、脂肪、氨基酸等营养物质代谢紊乱。含量发生显著变化的代谢物可能成为PCBs与高脂饮食暴露的候选毒性标志物。
목적：분석다록련분（ PCBs）여고지음식폭로후SD대서뇨액대사조변화，탐토PCBs대당、지방등대사적영향급가능적독성궤제，병심조잠재적후선생물표지물。방법웅성SD대서수궤분위대조조、고지조、PCBs조、PCBs여고지연합폭로조。우염독6주수집뇨액양본，채집뇨액적1 H NMR보，채용주성분분석법（ PCA）분석수거。결과 PCA득분도현시，연합폭로조여기타3조가완전구분，표명해조동물뇨액대사특정여기타3조존재교대차이。 PCA재하도현시，연합폭로조대서뇨액중유산、포도당、기산、2-간이무산함량현저상승；저몽산、호박산、우광산、마뇨산、양화삼갑알（ TMAO）함량현저하강。결론 PCBs여고지연합폭로도치대서뇨액중삼최산순배적중간산물함량강저혹여능량대사상관적대사물축적，가능여선립체공능수손、능량대사장애유관。저사효응가능회진일보도치당류、지방、안기산등영양물질대사문란。함량발생현저변화적대사물가능성위PCBs여고지음식폭로적후선독성표지물。
Objective To explore the effect on metabolism of glucose and lipids, potential toxicity mechanism and possible biomarker candidates by analyzing urine metabonome changes of rats after oral administration of polychlorinated biphenyl congeners( PCBs) and high fat diet alone or in combination.Methods Male SD rats were divided randomly into control group,high fat diet group, PCBs group and combination group of PCBs and high fat diet.Urine samples were collected after 6-week treatment, 1 H NMR spectra were performed and analyzed by principal component analysis ( PCA) . Results The PCA scores plot of urine 1 H NMR data showed that the combined group could be easily distinguished from the other three groups, suggesting great difference in metabolism.The loading plot of the PCA revealed significant increase in the levels of lactate, glucose, creatine, 2-hydroxy-isovaleric acid and reduction in the levels of citrate, succinate, taurine, hippurate and trimethylamine oxide ( TMAO) in the combined exposure group after six-week exposure.Conclusion The altered levels of metabolites induced by combined exposure of PCBs and high fat diet may be related to the injury to mitochondrial function, reduction of energy metabolism in tricarboxylic acid cycle (TAC).These effects possibly lead to perturbations in the metabolism of glucose, lipid and amino acids.The altered metabolites may be considered biomarker candidates of toxicity induced by exposure to PCBs and high fat diet.