CAJ | 학술논문

病原微生物检测一直是微生物学研究领域的热点，常规检测方法，如分离培养、PCR、ELISA和基因组测序等，样品前处理复杂，耗时较长，不利于现场和实时在线检测。光谱技术因具有快速准确、安全高效、非破坏性和便于在线侦检等特点，成为一种新型无创检测技术，在病原微生物检测、疾病诊断等领域得到迅速发展。该文就3种常用的光谱技术，即激光激发荧光光谱、红外光谱和拉曼光谱在病原微生物检测中的工作原理、研究进展作一综述，讨论各自优缺点，并展望其应用前景。
병원미생물검측일직시미생물학연구영역적열점，상규검측방법，여분리배양、PCR、ELISA화기인조측서등，양품전처리복잡，모시교장，불리우현장화실시재선검측。광보기술인구유쾌속준학、안전고효、비파배성화편우재선정검등특점，성위일충신형무창검측기술，재병원미생물검측、질병진단등영역득도신속발전。해문취3충상용적광보기술，즉격광격발형광광보、홍외광보화랍만광보재병원미생물검측중적공작원리、연구진전작일종술，토론각자우결점，병전망기응용전경。
Detection of pathogenic microorganisms has been a hot research field of microbiology.Conventional detection methods,such as isolation and culture, PCR technology, ELISA and genomic sequencing,are all time-consuming and com-plex.Because of the advantages of quick-testing, accuracy, safety and efficiency, spectroscopy has become a new non-inva-sive testing technology and has witnessed rapid development in pathogen detection and disease diagnosis.This article intro-duces three types of common spectroscopy technologies ( laser excitation fluorescence spectroscopy, infrared spectroscopy and Raman spectroscopy) , and also explains how they work in the detection of pathogenic microorganisms.