CAJ | 학술논문

目的：研制一种新型的拉曼增强液态基底，采用免标记表面增强拉曼散射（ surface-enhanced Raman scat-tering，SERS ）技术快速检测大肠杆菌。方法采用改进后的Stober方法制备360 nm二氧化硅纳米球（ SiO2），在其表面结合不同粒径Au＠Ag，制备纳米复合物（ SiO2-Au＠Ag）。通过透射电镜（ TEM）、紫外可见（ UV-Vis）分光光度仪对所制备的纳米结构进行分析表征。以对巯基苯胺（ PATP）为待检分子，筛选出SERS效果最佳的SiO2-Au＠Ag。以此纳米复合物检测不同浓度PATP，并通过简单混合培养的方法检测大肠杆菌。结果透射电镜拍摄图片显示，随着Au＠Ag 粒径增大，结合到SiO2表面的Au＠Ag 聚集度也增加。紫外光谱显示，随着Au＠Ag 粒径增大， Au＠Ag和SiO2-Au＠Ag最大吸收峰红移。通过实验，SiO2-100 nm Au＠Ag检测PATP的灵敏度为10-10 mol／L，最低可检测的大肠杆菌的浓度为105 CFU／ml。结论在该实验范围内，SiO2-Au＠Ag的SERS效应随着SiO2表面结合的Au＠Ag粒径增大而增大。 SiO2-100 nm Au＠Ag具有最强的SERS效应。
목적：연제일충신형적랍만증강액태기저，채용면표기표면증강랍만산사（ surface-enhanced Raman scat-tering，SERS ）기술쾌속검측대장간균。방법채용개진후적Stober방법제비360 nm이양화규납미구（ SiO2），재기표면결합불동립경Au＠Ag，제비납미복합물（ SiO2-Au＠Ag）。통과투사전경（ TEM）、자외가견（ UV-Vis）분광광도의대소제비적납미결구진행분석표정。이대구기분알（ PATP）위대검분자，사선출SERS효과최가적SiO2-Au＠Ag。이차납미복합물검측불동농도PATP，병통과간단혼합배양적방법검측대장간균。결과투사전경박섭도편현시，수착Au＠Ag 립경증대，결합도SiO2표면적Au＠Ag 취집도야증가。자외광보현시，수착Au＠Ag 립경증대， Au＠Ag화SiO2-Au＠Ag최대흡수봉홍이。통과실험，SiO2-100 nm Au＠Ag검측PATP적령민도위10-10 mol／L，최저가검측적대장간균적농도위105 CFU／ml。결론재해실험범위내，SiO2-Au＠Ag적SERS효응수착SiO2표면결합적Au＠Ag립경증대이증대。 SiO2-100 nm Au＠Ag구유최강적SERS효응。
Objective To develop a new type of Raman-enhanced substrate for rapid detection of E.coli based on label-free surface-enhanced Raman scattering( SERS) technology.Methods Stober’ s improved method was used to prepare 360 nm silica ( SiO2 ) nanospheres.Prepared gold core-silver shell nanoparticles( Au@Ag) of different size were attached to 360 nm SiO2 to fabricate the nanocomposites ( SiO2-Au@Ag ) that were characterized by transmission electron microscopy (TEM) and UV-visucl light adsorption spectra (UV-Vis).PATP was detected to select SiO2-Au@Ag with optimal SERS effect.This optimal SiO2-Au@Ag was used to obtain the sensitivity of PATP and E.coli detection after a simple mixed culti-vation.Results TEM images showed that Au@Ag aggregated with the size of Au@Ag attached to 360 nm SiO2 .UV-Vis spectra indicated that the maximum absorption of Au@Ag and SiO2-Au@Ag had a red shift with the invrease of Au@Ag size.The experiment results suggested that detection sensitivity of PATP by SiO2-100 nm Au@Ag 10 -10 mol/L, while the lowest detectable E.coli concentration was 105 CFU/ml.Conclusion The 360 nm SiO2 binding with 100nm Au@Ag exhibits great potential for SERS applications.