中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
5期
863-864,865
,共3页
张胜娜%吴素香%薛海娜%姜茜
張勝娜%吳素香%薛海娜%薑茜
장성나%오소향%설해나%강천
小儿双解止泻颗粒%葛根素%黄芩苷%高效液相色谱法
小兒雙解止瀉顆粒%葛根素%黃芩苷%高效液相色譜法
소인쌍해지사과립%갈근소%황금감%고효액상색보법
Xiao’ er Shuangjie Zhixie granules%Pueratin%Baicalin%HPLC
目的：：建立HPLC法同时测定小儿双解止泻颗粒中葛根素和黄芩苷含量的方法。方法：色谱粒为Agilent Zorbax SB-C18柱(250 mm ×4.6 mm,5μm)；流动相A为0.1%的磷酸溶液,流动相B为甲醇,二元梯度洗脱；检测波长：265 nm；流速：10 ml·min-1；柱温：30℃；进样量20ml。结果：葛根素进样量在0.121~1.942μg范围内(r=0.9995),黄芩苷进样量在0.230~3.686μg范围内线性关系良好(r=0.9995)；平均加样回收率分别为98.10%和98.20%,RSD分别为1.34%和1.11%(n=6)。结论：该法简便、快速、准确,可用于小儿双解止泻颗粒的质量控制。
目的：：建立HPLC法同時測定小兒雙解止瀉顆粒中葛根素和黃芩苷含量的方法。方法：色譜粒為Agilent Zorbax SB-C18柱(250 mm ×4.6 mm,5μm)；流動相A為0.1%的燐痠溶液,流動相B為甲醇,二元梯度洗脫；檢測波長：265 nm；流速：10 ml·min-1；柱溫：30℃；進樣量20ml。結果：葛根素進樣量在0.121~1.942μg範圍內(r=0.9995),黃芩苷進樣量在0.230~3.686μg範圍內線性關繫良好(r=0.9995)；平均加樣迴收率分彆為98.10%和98.20%,RSD分彆為1.34%和1.11%(n=6)。結論：該法簡便、快速、準確,可用于小兒雙解止瀉顆粒的質量控製。
목적：：건립HPLC법동시측정소인쌍해지사과립중갈근소화황금감함량적방법。방법：색보립위Agilent Zorbax SB-C18주(250 mm ×4.6 mm,5μm)；류동상A위0.1%적린산용액,류동상B위갑순,이원제도세탈；검측파장：265 nm；류속：10 ml·min-1；주온：30℃；진양량20ml。결과：갈근소진양량재0.121~1.942μg범위내(r=0.9995),황금감진양량재0.230~3.686μg범위내선성관계량호(r=0.9995)；평균가양회수솔분별위98.10%화98.20%,RSD분별위1.34%화1.11%(n=6)。결론：해법간편、쾌속、준학,가용우소인쌍해지사과립적질량공제。
Objective:To establish a method for the determination of puerarin and balcalin in Xiao’ er Shuangjie Zhixie granules by HPLC. Methods:An Agilent Zorbax SB-C18 column (250 mm × 4. 6 mm,5 μm) was used. The mobile phase A was 0. 1% phos-phoric acid solution and the mobile phase B was methanol with gradient elution. The detection wavelength was 265 nm,the flow rate was 1. 0 ml·min-1 ,the column temperature was 30℃ and the sample size was 20 μl. Results: The linear range of pueratin was 0. 121-1. 942 μg (r=0. 999 5)and that of balcalin was 0. 230-3. 686 μg(r=0. 999 5). The average recovery was 98. 10% and 98. 20%with RSD of 1. 34% and 1. 11%(n=6), respectively. Conclusion:The method is convenient and accurate, which can be used in the de-termination of pueraln and balcalin in Xiao’ er Shuangjie Zhixie granules.
