中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
5期
741-743,744
,共4页
神农茶颗粒%夏佛塔苷%异夏佛塔苷%去氧地胆草内酯%4 ,5-二咖啡酰奎宁酸%广金钱草%地胆草
神農茶顆粒%夏彿塔苷%異夏彿塔苷%去氧地膽草內酯%4 ,5-二咖啡酰奎寧痠%廣金錢草%地膽草
신농다과립%하불탑감%이하불탑감%거양지담초내지%4 ,5-이가배선규저산%엄금전초%지담초
Shennongcha granules%Schaftoside%Isoschaftoside%Deoxyelephantopin%4,5-Dicaffeoylquinic acid%Desmodii styra-cifolii herba%Elephantopi herba
目的：：建立同时测定神农茶颗粒中夏佛塔苷、异夏佛塔苷、去氧地胆草内酯、4,5-二咖啡酰奎宁酸4个成分的高效液相色谱测定方法。方法：采用Hypersil C18色谱柱(200 mm ×4.6 mm,5μm)；以乙腈-0.025 mol·L-1磷酸溶液为流动相进行梯度洗脱,体积流量：1.3 ml·min-1,检测波长：270 nm(夏佛塔苷和异夏佛塔苷)、208 nm(去氧地胆草内酯)、327 nm(4,5-二咖啡酰奎宁酸),柱温为室温。结果：夏佛塔苷、异夏佛塔苷、去氧地胆草内酯和4,5-二咖啡酰奎宁酸分别在5.850~117.000,4.650~93.000,4.160~83.200,5.470~109.400μg · ml-1范围内线性良好, r 值均大于0.999,平均加样回收率分别为97.70%、96.87%、97.53%、99.29%,RSD分别为1.40%、1.13%、1.69%、1.01%(n=6)。结论：该方法简便,结果准确,可用于神农茶颗粒的含量测定。
目的：：建立同時測定神農茶顆粒中夏彿塔苷、異夏彿塔苷、去氧地膽草內酯、4,5-二咖啡酰奎寧痠4箇成分的高效液相色譜測定方法。方法：採用Hypersil C18色譜柱(200 mm ×4.6 mm,5μm)；以乙腈-0.025 mol·L-1燐痠溶液為流動相進行梯度洗脫,體積流量：1.3 ml·min-1,檢測波長：270 nm(夏彿塔苷和異夏彿塔苷)、208 nm(去氧地膽草內酯)、327 nm(4,5-二咖啡酰奎寧痠),柱溫為室溫。結果：夏彿塔苷、異夏彿塔苷、去氧地膽草內酯和4,5-二咖啡酰奎寧痠分彆在5.850~117.000,4.650~93.000,4.160~83.200,5.470~109.400μg · ml-1範圍內線性良好, r 值均大于0.999,平均加樣迴收率分彆為97.70%、96.87%、97.53%、99.29%,RSD分彆為1.40%、1.13%、1.69%、1.01%(n=6)。結論：該方法簡便,結果準確,可用于神農茶顆粒的含量測定。
목적：：건립동시측정신농다과립중하불탑감、이하불탑감、거양지담초내지、4,5-이가배선규저산4개성분적고효액상색보측정방법。방법：채용Hypersil C18색보주(200 mm ×4.6 mm,5μm)；이을정-0.025 mol·L-1린산용액위류동상진행제도세탈,체적류량：1.3 ml·min-1,검측파장：270 nm(하불탑감화이하불탑감)、208 nm(거양지담초내지)、327 nm(4,5-이가배선규저산),주온위실온。결과：하불탑감、이하불탑감、거양지담초내지화4,5-이가배선규저산분별재5.850~117.000,4.650~93.000,4.160~83.200,5.470~109.400μg · ml-1범위내선성량호, r 치균대우0.999,평균가양회수솔분별위97.70%、96.87%、97.53%、99.29%,RSD분별위1.40%、1.13%、1.69%、1.01%(n=6)。결론：해방법간편,결과준학,가용우신농다과립적함량측정。
Objective:To establish an HPLC method for determining four constituents ( schaftoside, isoschaftoside, deoxyelephan-topin and 4,5-dicaffeoylquinic acid) in Shennongcha granules. Methods:An HPLC method was performed on a Hypersil C18 column (200 mm × 4. 6 mm,5 μm) with the mobile phase of acetonitrile as the phase A and 0. 025 mol·L-1 phosphoric acid solution as the phase B with gradient elution. The flow rate was 1. 3 ml·min-1 . The detection wavelength was set at 270 nm for schaftoside and isos-chaftoside, 208 nm for deoxyelephantopin and 327 nm for 4, 5-dicaffeoylquinic acid. The column temperature was room temperature. Results:The calibration curve was linear over the concentration range of 5. 850-117. 000 μg·ml-1 for schaftoside, 4. 650-93. 000 μg ·ml-1 for isoschaftoside, 4. 160-83. 200 μg · ml-1 for deoxyelephantopin and 5. 470-109. 400 μg · ml-1 for 4, 5-dicaffeoylquinic acid. The correlation coefficient of all curves was more than 0.999. The average recoverywas 97.70% (RSD=1.40%), 96.87%(RSD=1.13%), 97.53%(RSD =1.69%) and 99.29%(RSD =1.01%) (n =6) , respectively. Conclusion: The developed HPLC method is simple,accurate,and can be used in the content determination of Shennongcha granules.
