CAJ | 학술논문

目的：研究左旋门冬酰胺酶对急性T淋巴细胞白血病jurkat细胞株生长的影响。方法：在倒置相差显微镜下观察jurkat细胞经左旋门冬酰胺酶处理前后的生长状态，Gimsa染色观察L-Asp处理前后jurkat细胞的形态变化。通过琼脂糖凝胶电泳检测经不同浓度左旋门冬酰胺酶处理后的jurkat细胞的DNA片段。用流式细胞术检测jurkat细胞经不同浓度门冬酰胺酶处理48小时后的凋亡率及死亡率。结果：终浓度为1IU/ml、2.5IU/ml、5IU/ml、10IU/ml L-Asp均可诱导jurkat细胞发生凋亡和死亡，L-Asp终浓度为2.5IU/ml时jurkat细胞凋亡率最高，死亡率随L-Asp浓度增高而升高；凋亡细胞基因组DNA片段化断裂，凝胶电泳可见梯状条带。结论：左旋门冬酰胺酶可以诱导jurkat细胞发生凋亡，其凋亡细胞DNA发生片段化断裂，凋亡率与左旋门冬酰胺酶浓度有关。
목적：연구좌선문동선알매대급성T림파세포백혈병jurkat세포주생장적영향。방법：재도치상차현미경하관찰jurkat세포경좌선문동선알매처리전후적생장상태，Gimsa염색관찰L-Asp처리전후jurkat세포적형태변화。통과경지당응효전영검측경불동농도좌선문동선알매처리후적jurkat세포적DNA편단。용류식세포술검측jurkat세포경불동농도문동선알매처리48소시후적조망솔급사망솔。결과：종농도위1IU/ml、2.5IU/ml、5IU/ml、10IU/ml L-Asp균가유도jurkat세포발생조망화사망，L-Asp종농도위2.5IU/ml시jurkat세포조망솔최고，사망솔수L-Asp농도증고이승고；조망세포기인조DNA편단화단렬，응효전영가견제상조대。결론：좌선문동선알매가이유도jurkat세포발생조망，기조망세포DNA발생편단화단렬，조망솔여좌선문동선알매농도유관。
ObjectiveTo study the effects of L-asparaginase on the proliferation and apoptosis of human acute T-cell leukemia Jurkatcells.Method The morphological changes were observed under a microscope with Gimsa stained cells. DNA fragments were detected by Agarose gel electrophoresis. Cell apoptosis was analysed by Flow cytometry .ResultsThe jurkat cells , treated with L-Asp in the concentration of 1IU/ml、2.5IU/ml、5IU/ml、10IU/ml for 48h , can be induced apoptosis and death. The jurkat cells’ apoptosis rate was highest when treated with the concentration of L-Asp was 2.5IU/ml. The jurkat cells’ mortality rates increased with L-Asp concentration riseing. DNA ladder can be showed by DNA electrophoresis after the jurkat cells were treated with 1IU/ml、2.5IU/ml、5IU/ml、10IU/ml L-Asp.ConclusionsThe apoptosis of Jurkat cells can be induced by L-Asp. Apoptotic DNA occurs gragmentation. Apoptosis rate is associated with Concentration of L-Asp.