中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
5期
733-735,736
,共4页
车晓侠%席枝侠%王娜%赵宇%蔡艳%常权
車曉俠%席枝俠%王娜%趙宇%蔡豔%常權
차효협%석지협%왕나%조우%채염%상권
化生平胶囊%薄层色谱法%高效液相色谱法
化生平膠囊%薄層色譜法%高效液相色譜法
화생평효낭%박층색보법%고효액상색보법
Huashengping capsules%TLC%HPLC
目的：：建立化生平胶囊的质量标准。方法：采用TLC法对黄芪、白花蛇舌草、丹参进行鉴别；用HPLC法测定黄芪甲苷的含量,色谱柱为Kromasil C18(250 mm ×4.6 mm,5μm),流动相为乙腈-水(32∶68),流速：1.0 ml·min-1,检测波长：203 nm柱温：25℃,进样量：20μl。结果：TLC色谱斑点清晰；黄芪甲苷在进样量为2.000~10.000μg 范围内线性关系良好( r=0.9996),平均回收率为100.8%,RSD为1.9%(n=6)。结论：该方法简便、准确、可靠,可用于化生平胶囊的质量控制。
目的：：建立化生平膠囊的質量標準。方法：採用TLC法對黃芪、白花蛇舌草、丹參進行鑒彆；用HPLC法測定黃芪甲苷的含量,色譜柱為Kromasil C18(250 mm ×4.6 mm,5μm),流動相為乙腈-水(32∶68),流速：1.0 ml·min-1,檢測波長：203 nm柱溫：25℃,進樣量：20μl。結果：TLC色譜斑點清晰；黃芪甲苷在進樣量為2.000~10.000μg 範圍內線性關繫良好( r=0.9996),平均迴收率為100.8%,RSD為1.9%(n=6)。結論：該方法簡便、準確、可靠,可用于化生平膠囊的質量控製。
목적：：건립화생평효낭적질량표준。방법：채용TLC법대황기、백화사설초、단삼진행감별；용HPLC법측정황기갑감적함량,색보주위Kromasil C18(250 mm ×4.6 mm,5μm),류동상위을정-수(32∶68),류속：1.0 ml·min-1,검측파장：203 nm주온：25℃,진양량：20μl。결과：TLC색보반점청석；황기갑감재진양량위2.000~10.000μg 범위내선성관계량호( r=0.9996),평균회수솔위100.8%,RSD위1.9%(n=6)。결론：해방법간편、준학、가고,가용우화생평효낭적질량공제。
Objective:To establish the quality standard for Huashengping capsules. Methods: Milkvetch Root, Hedyotis diffusa Willd and Salvia miltiorrhiza were identified by TLC. The content of astragaloside A was detected by HPLC. The column was Kormasil C18(250 mm ×4.6 mm, 5 μm) and the flow rate was 1.0 ml·min-1. The mobile phases was a mixture of acetonitrile-water (32∶68 ) . The detection wavelength was 203 nm. The column temperature was 25℃ and the sample size was 20μl. Results:The TLC chro-matography spots were clear. Astragaloside A was linear within the range of 2. 000-10. 000 μg(r=0. 999 6) and the average recovery was 100. 8%(RSD=1. 9%,n=6). Conclusion:The method is simple, accurate and reliable, which can be used in the quality con-trol of Huashengping capsules.
