CAJ | 학술논문

目的：：研究新鲜水菖蒲根茎水提物的创伤促愈和抗炎活性。方法：应用切创模型考察水菖蒲创伤愈合作用,以图像分析技术和免疫组化进行分析,应用脂多糖(LPS)制备RAW264.7细胞系炎症模型,通过RT-PCR技术检测炎症因子水平,从而考察其抗炎活性。结果：于动物创伤模型日3次给药,3 d起给药组较对照组有创面面积减少,13 d后仅为对照组面积的15%,显著提高创伤治愈率,同时由脂多糖诱导的RAW 264.7炎症可被水菖蒲水提物有效抑制,给药组与对照组相比炎症因子TNF-α, IL-1β, IL-6的mRNA表达均下调,并呈现一定的剂量依赖性。结论：自新鲜水菖蒲根部的水提物在小鼠切创模型上表现出明显的促愈作用,同时在LPS诱导的炎症模型上体现了明显的抗炎作用。
목적：：연구신선수창포근경수제물적창상촉유화항염활성。방법：응용절창모형고찰수창포창상유합작용,이도상분석기술화면역조화진행분석,응용지다당(LPS)제비RAW264.7세포계염증모형,통과RT-PCR기술검측염증인자수평,종이고찰기항염활성。결과：우동물창상모형일3차급약,3 d기급약조교대조조유창면면적감소,13 d후부위대조조면적적15%,현저제고창상치유솔,동시유지다당유도적RAW 264.7염증가피수창포수제물유효억제,급약조여대조조상비염증인자TNF-α, IL-1β, IL-6적mRNA표체균하조,병정현일정적제량의뢰성。결론：자신선수창포근부적수제물재소서절창모형상표현출명현적촉유작용,동시재LPS유도적염증모형상체현료명현적항염작용。
Objective:To explore the wound-healing effect and the anti-inflammatory activity of the aqueous extracts from the fresh roots and rhizomes of A. calamus. Methods: The image analysis techniques and the histological analysis were used to determine the wound-healing effect in the excised wound test, and the real-time RT-PCR techniques was used to evaluate the anti-inflammatory activi-ty in the lipopolysaccharide-induced RAW 264. 7 cells test. Results:The aqueous extracts were given 3 times a day since the model was established. The skin wound area was reduced significantly in the aqueous extracts group when compared with that in the control group since the 3rd day, the wound area in the aqueous extracts group was only 15% of that in the control group on the 13th day, and the wound-healing rate was enhanced significantly by the extracts. Moreover, the mRNA expressions of the inflammatory mediators such as TNF-α, IL-1β and IL-6 in the inflammatory RAW 264. 7 cells induced by lipopolysaccharide were inhibited effectively by the ex-tracts in a dose-dependant manner. Conclusion:The results indicate that the aqueous extracts from the fresh roots and rhizomes of A. calamus have significant wound-healing activity in animal excised wound model and anti-inflammatory activity in vitro.