CAJ | 학술논문

目的：以核糖体转录间隔区(rDNA ITS)序列为分子标记，对1种产自贵州安顺的华钩藤植物进行遗传分析与分子鉴定。方法：通过改良CTAB法提取样品总DNA，利用通用引物对rDNA ITS序列进行PCR扩增，经克隆、测序后，运用Clustal X、BioEdit和PAUP等软件进行序列分析和构建系统发育树。结果：测序得到样品的rDNA ITS区序列长度为719bp，序列分析结果显示其与Genbank中已有的华钩藤[Uncaria sinensi(Oliv.)Havil.] rDNA ITS区序列之间相似性达99.8%，并且在系统发育树中并排聚类成1支。结论：基于rDNA ITS区序列的测序分析和系统发育树构建的分子生物学方法，能够对华钩藤植物进行准确的分子鉴定，为钩藤属中药材的种类鉴定和种间分类提供分子生物学理论依据。
목적：이핵당체전록간격구(rDNA ITS)서렬위분자표기，대1충산자귀주안순적화구등식물진행유전분석여분자감정。방법：통과개량CTAB법제취양품총DNA，이용통용인물대rDNA ITS서렬진행PCR확증，경극륭、측서후，운용Clustal X、BioEdit화PAUP등연건진행서렬분석화구건계통발육수。결과：측서득도양품적rDNA ITS구서렬장도위719bp，서렬분석결과현시기여Genbank중이유적화구등[Uncaria sinensi(Oliv.)Havil.] rDNA ITS구서렬지간상사성체99.8%，병차재계통발육수중병배취류성1지。결론：기우rDNA ITS구서렬적측서분석화계통발육수구건적분자생물학방법，능구대화구등식물진행준학적분자감정，위구등속중약재적충류감정화충간분류제공분자생물학이론의거。
Objective:To characterize the phylogenetic relationship of one Guizhou Uncaria sinensis(Oliv.)Havil,molecular identification and genetic analysis were carried out using the rDNA ITS sequence as molecular marker. Methods:Molecular identification and genetic analysis were carried out with the rDNA ITS sequence as molecular marker.Total DNA was extracted with modified CTAB Method:and there by rDNA ITS regions were amplified with primers ITS4/ITS5, sequenced and phylogenetic analysed with Clustal X, BioEdit and PAUP. Results:The entire rDNA ITS sequence of Uncaria was 719bp.Sequence analysis showed that the rDNA ITS sequence was closely related to Uncaria sinensis(Oliv. ) Havil available in GenBank and the similarity reached 99.8%. Conclusion Based on molecular biology Methods:of rDNA ITS region analysis, molecular identification is useful inaccurate classification on Uncaria sinensis(Oliv.)Havil.,which provides molecular evidence of taxonomy and identification of different species in genus Uncaria.