湖泊科学
湖泊科學
호박과학
JOURNAL OF LAKE SCIENCES
2015年
3期
445-450
,共6页
沉积物%苦草%微生物群落结构%磷脂脂肪酸
沉積物%苦草%微生物群落結構%燐脂脂肪痠
침적물%고초%미생물군락결구%린지지방산
Sediments%Vallisneria natans%microbial community%PLFAs
模拟湖泊系统构建了“沉积物水苦草( Vallisneria natans)”系统,应用磷脂脂肪酸( PLFAs)法测定在沉水植物苦草不同生长时期沉积物表层微生物群落结构的变化,探讨沉水植物对沉积物中的微生物群落结构的影响.结果表明,从苦草生长初期到旺盛期再到衰亡期,沉积物中有机质含量先下降后上升;总磷、有机磷、无机磷分别下降了8.97%、7.81%、10.28%;沉积物微生物的活性与总磷呈极显著负相关,在苦草生长初期和旺盛期,实验组的沉积物微生物活性大于对照组,而在衰亡期对照组的沉积物微生物活性略高于实验组;不同时期沉积物中微生物群落结构发生了明显变化,组成结构差异显著,微生物组成中细菌占主要成分(占微生物总量的76%~84%);细菌中革兰氏阳性菌占主要优势,且革兰氏阳性菌百分含量随苦草生长呈上升趋势,革兰氏阴性菌呈下降趋势;真菌的百分含量呈上升趋势.
模擬湖泊繫統構建瞭“沉積物水苦草( Vallisneria natans)”繫統,應用燐脂脂肪痠( PLFAs)法測定在沉水植物苦草不同生長時期沉積物錶層微生物群落結構的變化,探討沉水植物對沉積物中的微生物群落結構的影響.結果錶明,從苦草生長初期到旺盛期再到衰亡期,沉積物中有機質含量先下降後上升;總燐、有機燐、無機燐分彆下降瞭8.97%、7.81%、10.28%;沉積物微生物的活性與總燐呈極顯著負相關,在苦草生長初期和旺盛期,實驗組的沉積物微生物活性大于對照組,而在衰亡期對照組的沉積物微生物活性略高于實驗組;不同時期沉積物中微生物群落結構髮生瞭明顯變化,組成結構差異顯著,微生物組成中細菌佔主要成分(佔微生物總量的76%~84%);細菌中革蘭氏暘性菌佔主要優勢,且革蘭氏暘性菌百分含量隨苦草生長呈上升趨勢,革蘭氏陰性菌呈下降趨勢;真菌的百分含量呈上升趨勢.
모의호박계통구건료“침적물수고초( Vallisneria natans)”계통,응용린지지방산( PLFAs)법측정재침수식물고초불동생장시기침적물표층미생물군락결구적변화,탐토침수식물대침적물중적미생물군락결구적영향.결과표명,종고초생장초기도왕성기재도쇠망기,침적물중유궤질함량선하강후상승;총린、유궤린、무궤린분별하강료8.97%、7.81%、10.28%;침적물미생물적활성여총린정겁현저부상관,재고초생장초기화왕성기,실험조적침적물미생물활성대우대조조,이재쇠망기대조조적침적물미생물활성략고우실험조;불동시기침적물중미생물군락결구발생료명현변화,조성결구차이현저,미생물조성중세균점주요성분(점미생물총량적76%~84%);세균중혁란씨양성균점주요우세,차혁란씨양성균백분함량수고초생장정상승추세,혁란씨음성균정하강추세;진균적백분함량정상승추세.
A sediment-water-Vallisneria natans system was built within a simulating lake system, where phospholipid fatty acids ( PLFAs) method was used to determine the changes in sediment microbial community structures in the submerged plant V. natans at different growth stages. The results showed that during the growth of V. natans, the organic matter content decreased first and then increased. The concentrations of total phosphorus, organic phosphorus and inorganic phosphorus decreased by 8. 97%, 7. 81% and 10. 28%, respectively, during the growth period. The microbial activity and total phosphorus in sediments showed ex-tremely significantly negative correlation. In the early exuberant period, microbial activity in the experimental group was greater than the control group, while the decline in the microbial activity of the control group was slightly higher than the experimental group. Microbial community structure in the sediments of different periods showed significant differences. Bacteria were the main part of the total sediment microbes, accounting for 76% to 84% of the total microbial. As to bacterial distribution, gram-positive bacilli were predominant. The percentage of gram-positive bacteria and fungi increased with the growth of the V. natans.