CAJ | 학술논문

目的：考察新疆部分地区市场销售的新疆紫草药材的质量。方法收集新疆不同地区市场上销售的新疆紫草药材10批，采用紫外分光光度法（药典法）测定羟基萘醌总色素含量，反相高效液相色谱法测定紫草中左旋紫草素、乙酰紫草素、去氧紫草素、β，β′-二甲基丙烯酰紫草素的含量。色谱条件：色谱柱 Scienhome Kromasil C18（4．6 mm ×200 mm，5μm），流动相为乙腈-0．3％磷酸，采用梯度洗脱，流速1．0 mL／min，柱温35℃，检验波长274，洗脱时间50 min。结果紫外分光光度法测得10批药材中羟基萘醌总色素含量为0．60～17．30 mg／g，高效液相色谱法测得左旋紫草素的含量为0．01～0．96 mg／g，乙酰紫草素含量为0．39～24．10 mg／g，去氧紫草素含量为0．77～31．10 mg／g，β，β′-二甲基丙烯酰紫草素含量为0．25～13．20 mg／g。结论新疆市场流通的新疆紫草药材羟基萘醌总色素及各成分含量差异很大。
목적：고찰신강부분지구시장소수적신강자초약재적질량。방법수집신강불동지구시장상소수적신강자초약재10비，채용자외분광광도법（약전법）측정간기내곤총색소함량，반상고효액상색보법측정자초중좌선자초소、을선자초소、거양자초소、β，β′-이갑기병희선자초소적함량。색보조건：색보주 Scienhome Kromasil C18（4．6 mm ×200 mm，5μm），류동상위을정-0．3％린산，채용제도세탈，류속1．0 mL／min，주온35℃，검험파장274，세탈시간50 min。결과자외분광광도법측득10비약재중간기내곤총색소함량위0．60～17．30 mg／g，고효액상색보법측득좌선자초소적함량위0．01～0．96 mg／g，을선자초소함량위0．39～24．10 mg／g，거양자초소함량위0．77～31．10 mg／g，β，β′-이갑기병희선자초소함량위0．25～13．20 mg／g。결론신강시장류통적신강자초약재간기내곤총색소급각성분함량차이흔대。
Objective To investigate the quality of Arnebia euchroma (Royle )J ohnst circulated in parts of Xingjian medicinal materials markets.Methods Ten batches of crude materials of Arnebia euchroma (Royle )J ohnst in different regions of Xinjiang were collected for the total contents measurement of hydroxynaphthoquinones by UV spectrophotometry and contents measurement of four major naphthoqui-nones:shikonin,acetylshikonin,deoxy shikonin andβ,β′-dimethylacryl shikonin by Re-HPLC simultane-ously with the chromatographic conditions,in which the Scienhome Kromasil C18 column (4.6 mm × 200 mm,5 μm),acetonitrile -0.3% phosphoric acid were used as mobile phase by gradient eluting,at 35℃ with the flow rate of 1.0 mL/min for 50 min,detecting at 274 nm.Results The total contents of hydroxynaphthoquinones in different samples were 0.6-17.3 mg/g by UV spectrophotometry,the content of shikonin,acetylshikonin,deoxy shikonin andβ,β′-dimethylacryl shikonin were 0.01-0.96 mg/g,0.39-24.1 mg/g,0.77-31.1 mg/g and 0.25-13.2 mg/g,respectively.Conclusion There were great differ-ences in contents of total hydroxyl naphthoquinone and four major naphthoquinones in Arnebia euchroma (Royle )J ohnst circulated in different parts of Xinjiang.