军事医学
軍事醫學
군사의학
BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES
2015年
4期
250-253
,共4页
张师%方以群%攸璞%姚健%李开诚%包晓辰
張師%方以群%攸璞%姚健%李開誠%包曉辰
장사%방이군%유박%요건%리개성%포효신
趋化因子受体4%AMD3100%氧中毒%肺组织%大鼠
趨化因子受體4%AMD3100%氧中毒%肺組織%大鼠
추화인자수체4%AMD3100%양중독%폐조직%대서
chemokine receptor 4%AMD3100%oxygen intoxication%lung tissues%rat
目的:研究趋化因子受体4(chemokine receptor 4,CXCR4)特异性拮抗剂AMD3100在大鼠肺型氧中毒( pulmonary oxygen intoxication)肺组织损伤中的干预作用。方法40只雄性SD大鼠随机分为常压空气PBS组、常压空气抑制剂组、纯氧暴露PBS组及纯氧暴露抑制剂组,每组10只。纯氧暴露PBS组及纯氧暴露抑制剂组利用0.23 MPa纯氧加压暴露,出舱后通过HE染色切片观察大鼠肺组织病理改变,ELISA法测定炎症因子TNF-α及IL-1β含量变化,应用Western印迹方法研究大鼠肺组织caspase-3含量变化。结果纯氧暴露抑制剂组大鼠肺组织淤血、水肿、出血与纯氧暴露PBS组相比程度较轻。纯氧暴露抑制剂组TNF-α、IL-1β及活化的caspase-3含量均低于纯氧暴露PBS组。结论应用AMD3100阻断CXCR4来减轻肺型氧中毒肺损伤是有效的。
目的:研究趨化因子受體4(chemokine receptor 4,CXCR4)特異性拮抗劑AMD3100在大鼠肺型氧中毒( pulmonary oxygen intoxication)肺組織損傷中的榦預作用。方法40隻雄性SD大鼠隨機分為常壓空氣PBS組、常壓空氣抑製劑組、純氧暴露PBS組及純氧暴露抑製劑組,每組10隻。純氧暴露PBS組及純氧暴露抑製劑組利用0.23 MPa純氧加壓暴露,齣艙後通過HE染色切片觀察大鼠肺組織病理改變,ELISA法測定炎癥因子TNF-α及IL-1β含量變化,應用Western印跡方法研究大鼠肺組織caspase-3含量變化。結果純氧暴露抑製劑組大鼠肺組織淤血、水腫、齣血與純氧暴露PBS組相比程度較輕。純氧暴露抑製劑組TNF-α、IL-1β及活化的caspase-3含量均低于純氧暴露PBS組。結論應用AMD3100阻斷CXCR4來減輕肺型氧中毒肺損傷是有效的。
목적:연구추화인자수체4(chemokine receptor 4,CXCR4)특이성길항제AMD3100재대서폐형양중독( pulmonary oxygen intoxication)폐조직손상중적간예작용。방법40지웅성SD대서수궤분위상압공기PBS조、상압공기억제제조、순양폭로PBS조급순양폭로억제제조,매조10지。순양폭로PBS조급순양폭로억제제조이용0.23 MPa순양가압폭로,출창후통과HE염색절편관찰대서폐조직병리개변,ELISA법측정염증인자TNF-α급IL-1β함량변화,응용Western인적방법연구대서폐조직caspase-3함량변화。결과순양폭로억제제조대서폐조직어혈、수종、출혈여순양폭로PBS조상비정도교경。순양폭로억제제조TNF-α、IL-1β급활화적caspase-3함량균저우순양폭로PBS조。결론응용AMD3100조단CXCR4래감경폐형양중독폐손상시유효적。
Objective To investigate the intervention of chemokine receptor 4(CXCR4) antagonist AMD3100 in lung tissues of rats during pulmonary oxygen intoxication.Methods Forty SD rats were randomly divided into 4 groups:normal pressure air PBS group, normal pressure air antagonist group , oxygen exposure PBS group and oxygen exposure antagonist group, each consisting of 10 animals.The last two groups were compressed to 0.23 MPa at an exponential rate of 0.1 MPa/min by pure oxygen.Pathological changes of lung tissues were observed by hematoxylin eosin stain.Changes in TNF-αand IL-1βexpression levels in the lung tissues of rats were detected by ELISA.Changes in CXCR4 expression levels were ob-served by Western blotting.Results Pathological examination indicated that edema and hemorrhage in the alveolar and pulmonary interstitial tissue of oxygen exposure antagonist group were lighter than in oxygen exposure PBS group.The levels of TNF-α, IL-1βand cleaved-caspase-3 in the lung tissues of the oxygen exposure antagonist group were lower than in oxy-gen exposure PBS group.Conclusion Blocking CXCR4 with AMD3100 can effectively alleviate lung injury during pulmo-nary oxygen intoxication.