化学与生物工程
化學與生物工程
화학여생물공정
CHEMISTRY & BIOENGINEERING
2015年
4期
26-29
,共4页
等电聚焦%pH 梯度%两性小分子%制备
等電聚焦%pH 梯度%兩性小分子%製備
등전취초%pH 제도%량성소분자%제비
isoelectric focusing%pH gradient%small zwitterionic compounds%preparation
建立了无需两性电解质的等电聚焦微量制备方法，可以分离两性小分子物质。采用混合酸性和碱性溶液在填充琼脂糖凝胶的色谱柱中制备了 pH 值梯度变化的缓冲溶液，上样后，在色谱柱上外加电场进行等电聚焦分离，随着终止缓冲溶液的连续加入，各种样品被聚焦在一定的 pH 值区域并依次洗脱，分段收集。考察了外加电场对 pH 梯度的影响以及外加电场、pH 梯度对等电聚焦分离的影响。模式样品的分离结果表明，所建立的方法可以实现两性小分子物质按照等电点顺序依次分离。
建立瞭無需兩性電解質的等電聚焦微量製備方法，可以分離兩性小分子物質。採用混閤痠性和堿性溶液在填充瓊脂糖凝膠的色譜柱中製備瞭 pH 值梯度變化的緩遲溶液，上樣後，在色譜柱上外加電場進行等電聚焦分離，隨著終止緩遲溶液的連續加入，各種樣品被聚焦在一定的 pH 值區域併依次洗脫，分段收集。攷察瞭外加電場對 pH 梯度的影響以及外加電場、pH 梯度對等電聚焦分離的影響。模式樣品的分離結果錶明，所建立的方法可以實現兩性小分子物質按照等電點順序依次分離。
건립료무수량성전해질적등전취초미량제비방법，가이분리량성소분자물질。채용혼합산성화감성용액재전충경지당응효적색보주중제비료 pH 치제도변화적완충용액，상양후，재색보주상외가전장진행등전취초분리，수착종지완충용액적련속가입，각충양품피취초재일정적 pH 치구역병의차세탈，분단수집。고찰료외가전장대 pH 제도적영향이급외가전장、pH 제도대등전취초분리적영향。모식양품적분리결과표명，소건립적방법가이실현량성소분자물질안조등전점순서의차분리。
Preparative ampholyte-carrier-free isoelectric focusing electrophoresis was established to separate small zwitterionic compounds.The pH gradient buffer solution was prepared by introducing a series of mixed acid and alkali solution at different pH value into a column containing agarose gel.After loading sample,addi-tional electric field was applied on the column to perform isoelectric focusing electrophoresis.With the continu-ous addition of last buffer solution,the samples were focused at certain pH value zone and eluted from the col-umn one by one,so that they could be fractionatedly collected.The effect of additional electric field on pH gra-dient was investigated.The effects of additional electric field and pH gradient on isoelectric focusing electropho-resis were also studied.Separation results of model samples revealed that small zwitterionic compounds could be separated according to the pI values by this method.
