CAJ | 학술논문

利用响应面法优化龙葵中澳洲茄边碱的提取工艺。采用单因素实验结合 Box-Behnken 中心组合设计，以澳洲茄边碱提取率为指标，考察乙醇体积分数、提取时间、料液比等因素对提取效果的影响。确定龙葵中澳洲茄边碱的最优提取工艺为：乙醇体积分数80％、提取时间2.1 h、料液比1∶12（g∶mL）、提取2次，在此条件下，澳洲茄边碱提取率为0.721 mg·g－1，与理论值（0.712 mg·g－1）相符，表明工艺可行，预测性较好。从龙葵中提取的澳洲茄边碱能明显抑制 K562、HL-60、BGC-823、MCF-7、Hela 等肿瘤细胞的增殖，具有较强的体外抗肿瘤活性。
이용향응면법우화룡규중오주가변감적제취공예。채용단인소실험결합 Box-Behnken 중심조합설계，이오주가변감제취솔위지표，고찰을순체적분수、제취시간、료액비등인소대제취효과적영향。학정룡규중오주가변감적최우제취공예위：을순체적분수80％、제취시간2.1 h、료액비1∶12（g∶mL）、제취2차，재차조건하，오주가변감제취솔위0.721 mg·g－1，여이론치（0.712 mg·g－1）상부，표명공예가행，예측성교호。종룡규중제취적오주가변감능명현억제 K562、HL-60、BGC-823、MCF-7、Hela 등종류세포적증식，구유교강적체외항종류활성。
Response surface methodology(RSM)was employed to optimize the extraction process of solama-rgine from Solanum nigrum L..Using extraction rate of solamargine as index,the effects of ethanol volume fraction,extraction time and solid-liquid ratio on extraction efficiency were investigated by single factor experi-ment and Box-Behnken central composite design.The optimum extraction conditions were obtained as follows:ethanol volume fraction 80%,extraction time 2.1 h,solid-liquid ratio 1 ∶ 12(g∶mL),extracting for 2 times. Under above conditions,the extraction rate was 0.721 mg· g-1 ,which was well met with the predictive of 0.712 mg·g-1 .Results showed that the process was feasible and had good predictability.Solamargine extracted from Solanum nigrum L.had obvious inhibitory effects on K562,HL-60,BGC-823,MCF-7 and Hela cell lines, and exhibited significant antitumor activities in vitro .