CAJ | 학술논문

采用直接回流法提取马齿苋中总黄酮，通过单因素实验与正交实验对提取工艺进行了优化；并以芦丁为对照品，采用分光光度法对马齿苋中的总黄酮含量进行了检测；同时研究了马齿苋总黄酮提取液对羟基自由基（·OH）和超氧阴离子自由基（·O－2）的清除效果，并与 Vc 的抗氧化活性进行了比较。确定马齿苋中总黄酮最优提取工艺为：提取时间50 min、水浴温度90℃、提取溶剂为30％乙酸乙酯、固液比1∶50（g∶mL），在此条件下，马齿苋总黄酮提取率可达7.15 mg·g－1。当马齿苋总黄酮浓度为0.18 mg·mL－1时，对· OH 的清除率高达73.12％，对· O－2的清除率可达49.02％，相同浓度时，其抗氧化活性低于 Vc。
채용직접회류법제취마치현중총황동，통과단인소실험여정교실험대제취공예진행료우화；병이호정위대조품，채용분광광도법대마치현중적총황동함량진행료검측；동시연구료마치현총황동제취액대간기자유기（·OH）화초양음리자자유기（·O－2）적청제효과，병여 Vc 적항양화활성진행료비교。학정마치현중총황동최우제취공예위：제취시간50 min、수욕온도90℃、제취용제위30％을산을지、고액비1∶50（g∶mL），재차조건하，마치현총황동제취솔가체7.15 mg·g－1。당마치현총황동농도위0.18 mg·mL－1시，대· OH 적청제솔고체73.12％，대· O－2적청제솔가체49.02％，상동농도시，기항양화활성저우 Vc。
Total flavonoids were extracted from purslane by direct reflux.The extraction process was opti-mized by single factor experiment and orthogonal experiment.The content of total flavonoids from purslane was determined by spectrophotometric method using rutin as reference substance.Scavenging efficiency of total fla-vonoids from purslane to ·OH and ·O-2 were studied and compared with that of Vc.The optimal extraction process was obtained as follows:extraction time of 50 min,water bath temperature of 90 ℃,30% ethyl acetate as extraction solvent,and solid-liquid ratio of 1∶50(g∶mL).Under the optimal conditions,the extraction rate of total flavonoids from purslane could reach as high as 7.15 mg· g-1 .When the concentration of total fla-vonoids from purslane was 0.18 mg·mL-1 ,the scavenging rate of ·OH was 73.12% and the scavenging rate of ·O-2 was 49.02%.The antioxidant activity of Vc was better than those of total flavonoids from purslane at the same concentration.