吉林医学
吉林醫學
길림의학
JILIN MEDICAL JOURANL
2015年
11期
2189-2191
,共3页
神经干细胞%Y27632%干细胞分化%Rho激酶抑制剂
神經榦細胞%Y27632%榦細胞分化%Rho激酶抑製劑
신경간세포%Y27632%간세포분화%Rho격매억제제
Neural stem cells%Y27632%Differentiation of stem cells%Rho kinase inhibitor
目的：探讨Rho激酶抑制剂Y-27632对神经干细胞增殖分化的影响。方法：分离培养大鼠神经干细胞并进行鉴定，设置对照组、LPA组、Y27632组，连续培养2周，观察细胞单克隆个数、单克隆形成率、Ki67和GFAP的基因表达量及蛋白质表达水平。结果：分离培养的神经干细胞经免疫荧光检测Nestin、CD133均为阳性；软琼脂实验发现，LPA组单克隆个数及单克隆形成率较对照组及Y27632组显著升高（P＜0.05），而Y27632组的单克隆个数及单克隆形成率与对照组相比差异无统计学意义（P＞0.05）；荧光定量及Western blotting结果显示，与对照组相比，LPA组GFAP基因表达量没明显变化（ P＞0.05），Ki67基因表达量升高（ P＜0.05），而Y27632组GFAP基因表达水平显著升高（ P＜0.05），而Ki67基因表达无变化（ P＞0.05）。结论：Rho激酶抑制剂Y27632可提高体外培养神经干细胞的GFAP表达水平，对于损伤神经细胞的修复具有重要作用。
目的：探討Rho激酶抑製劑Y-27632對神經榦細胞增殖分化的影響。方法：分離培養大鼠神經榦細胞併進行鑒定，設置對照組、LPA組、Y27632組，連續培養2週，觀察細胞單剋隆箇數、單剋隆形成率、Ki67和GFAP的基因錶達量及蛋白質錶達水平。結果：分離培養的神經榦細胞經免疫熒光檢測Nestin、CD133均為暘性；軟瓊脂實驗髮現，LPA組單剋隆箇數及單剋隆形成率較對照組及Y27632組顯著升高（P＜0.05），而Y27632組的單剋隆箇數及單剋隆形成率與對照組相比差異無統計學意義（P＞0.05）；熒光定量及Western blotting結果顯示，與對照組相比，LPA組GFAP基因錶達量沒明顯變化（ P＞0.05），Ki67基因錶達量升高（ P＜0.05），而Y27632組GFAP基因錶達水平顯著升高（ P＜0.05），而Ki67基因錶達無變化（ P＞0.05）。結論：Rho激酶抑製劑Y27632可提高體外培養神經榦細胞的GFAP錶達水平，對于損傷神經細胞的脩複具有重要作用。
목적：탐토Rho격매억제제Y-27632대신경간세포증식분화적영향。방법：분리배양대서신경간세포병진행감정，설치대조조、LPA조、Y27632조，련속배양2주，관찰세포단극륭개수、단극륭형성솔、Ki67화GFAP적기인표체량급단백질표체수평。결과：분리배양적신경간세포경면역형광검측Nestin、CD133균위양성；연경지실험발현，LPA조단극륭개수급단극륭형성솔교대조조급Y27632조현저승고（P＜0.05），이Y27632조적단극륭개수급단극륭형성솔여대조조상비차이무통계학의의（P＞0.05）；형광정량급Western blotting결과현시，여대조조상비，LPA조GFAP기인표체량몰명현변화（ P＞0.05），Ki67기인표체량승고（ P＜0.05），이Y27632조GFAP기인표체수평현저승고（ P＜0.05），이Ki67기인표체무변화（ P＞0.05）。결론：Rho격매억제제Y27632가제고체외배양신경간세포적GFAP표체수평，대우손상신경세포적수복구유중요작용。
Objective To explore the effect of Rho kinase inhibitor Y-27632 on proliferation and differentiation of neural stem cells. Method The neural stem cells of the rats were isolated ,cultured and identified. Blank control group,LPA treatment group and group Y27632 were set and cultured for two weeks. The number of monoclonal cells and the gene and protein expression level of Ki67 and GFAP were observed. Results The isolated and cultured neural stem cells were tested by immunofluorescence detection showing that Nestin and CD133 were positive;It was found in soft agar assay that the monoclonal number of group LPA was the most while there was no significant difference between the group Y27632 and the control group(P>0. 05);The results of fluorescent quantitation and western blotting showed that compared with the control group,the expression level of GFAP in group LPA,did not change obviously( P>0. 05),the expression level of Ki67 elevated while the gene expression level of group Y27632 increased significantly but the gene expression of Ki67 had no changes( P<0. 05). Conclusion Rho kinase inhibitor Y27632 can raise the expression level of GFAP of neural stem cells in vitro culture and plays an important role in the repair of the damaged nerve cells.
