陕西理工学院学报(自然科学版)
陝西理工學院學報(自然科學版)
협서리공학원학보(자연과학판)
JOURNAL OF SHAANXI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE EDITION)
2015年
2期
62-65
,共4页
盘龙七%秦岭岩白菜%岩白菜素%含量分析
盤龍七%秦嶺巖白菜%巖白菜素%含量分析
반룡칠%진령암백채%암백채소%함량분석
Rhizoma Bergeniae Scopulosae%Bergenia scopulosa T.P.Wang%bergenin%contents analysis
利用高效液相法测定14个不同产地盘龙七中岩白菜素的含量。色谱条件:色谱柱为Inertsil ODS-3C18(4.6 mm ×150 mm,5μm)柱,乙腈-0.2%磷酸溶液(9∶91)为流动相,流速1.0 mL/min,检测波长275 nm,柱温30℃。岩白菜素在15~75μg/mL范围内呈良好的线性关系, Y =14942.0X -428.6(R2=0.9998),平均加样回收率100.58%,RSD为1.77%( n=6)。分析结果表明,不同产地盘龙七中岩白菜素的含量不同,其含量随产地的不同而显示出一定的差异,其变化范围在14.68~70.19 mg/g,含量较为丰富。本研究建立盘龙七药材中岩白菜素含量测定方法,并分析检测了产自陕西、甘肃、四川三省14个产地盘龙七中岩白菜素的含量,其结果可为盘龙七药材的质量控制提供科学依据。
利用高效液相法測定14箇不同產地盤龍七中巖白菜素的含量。色譜條件:色譜柱為Inertsil ODS-3C18(4.6 mm ×150 mm,5μm)柱,乙腈-0.2%燐痠溶液(9∶91)為流動相,流速1.0 mL/min,檢測波長275 nm,柱溫30℃。巖白菜素在15~75μg/mL範圍內呈良好的線性關繫, Y =14942.0X -428.6(R2=0.9998),平均加樣迴收率100.58%,RSD為1.77%( n=6)。分析結果錶明,不同產地盤龍七中巖白菜素的含量不同,其含量隨產地的不同而顯示齣一定的差異,其變化範圍在14.68~70.19 mg/g,含量較為豐富。本研究建立盤龍七藥材中巖白菜素含量測定方法,併分析檢測瞭產自陝西、甘肅、四川三省14箇產地盤龍七中巖白菜素的含量,其結果可為盤龍七藥材的質量控製提供科學依據。
이용고효액상법측정14개불동산지반룡칠중암백채소적함량。색보조건:색보주위Inertsil ODS-3C18(4.6 mm ×150 mm,5μm)주,을정-0.2%린산용액(9∶91)위류동상,류속1.0 mL/min,검측파장275 nm,주온30℃。암백채소재15~75μg/mL범위내정량호적선성관계, Y =14942.0X -428.6(R2=0.9998),평균가양회수솔100.58%,RSD위1.77%( n=6)。분석결과표명,불동산지반룡칠중암백채소적함량불동,기함량수산지적불동이현시출일정적차이,기변화범위재14.68~70.19 mg/g,함량교위봉부。본연구건립반룡칠약재중암백채소함량측정방법,병분석검측료산자협서、감숙、사천삼성14개산지반룡칠중암백채소적함량,기결과가위반룡칠약재적질량공제제공과학의거。
The contents of bergenin in Rhizoma Bergeniae Scopulosae from different areas were deter-mined by using HPLC.The chromatographic separation was carried out using Inertsil ODS-3 C18 column (4.6 mm ×150 mm,5 μm), the mobile phase was acetonitrile:0.2%phosphoric acid (9∶91), the detec-tion wavelength was set at 275 nm, the temperature was 30℃, and the flow rate was 1.0 mL/min.The cali-bration curve was linear in the range of 15 ~75 μg/mL and the regression equation for bergenin was Y =14 942.0X -428.6 (R2 =0.999 8) , the average recovery was 100.58% with RSD of 1.77%( n =6). The results showed that there are some differentces in the content of bergenin among different areas,and that the contents of bergenin were ranges betweenfrom 14.68~70.19 mg /g.This method is simple, efficient and accurate, which can be used for the analysis of bergenin in Rhizoma Bergeniae Scopulosae.