CAJ | 학술논문

目的观察慢性压迫性脊髓症大鼠模型脊髓基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)表达和血脊髓屏障(blood spinal cord barrier,BSCB)的变化,探讨二者相关性.方法将48只成年SD大鼠随机分为假手术组和脊髓压迫组,每组各24只;将吸水后可膨胀聚氨酯薄板植入C5椎板下硬膜外,建立慢性压迫性脊髓症模型.造模后第4周,两组分别通过BBB(Basso Beattie Bresnahan)评分和体感诱发电位(somatosensory evoked potential,SEP)检测评价脊髓功能,每组各取12只行MMP-9、内皮屏障抗原(endothelial barrier antigen,EBA)免疫组化染色、检测二者在脊髓不同区域表达情况;其余12只行伊文思蓝(evans blue,EB)灌注检测BSCB通透性.统计学方法比较两组各观察指标差异,并分析脊髓压迫组MMP-9与EBA表达变化的相关性.结果成功造模后第4周脊髓压迫组BBB评分显著低于假手术组;相对于假手术组,脊髓压迫组SEP检测潜伏期显著延长、波幅显著降低.脊髓压迫组白、灰质MMP-9表达均显著高于假手术组,脊髓压迫组灰质EBA表达显著低于假手术组,白质EBA表达无显著变化,脊髓压迫组EB含量显著高于假手术组;脊髓压迫组MMP-9表达与EBA表达半定量结果进行配对样本相关性分析显示两者呈显著相关(r=0.96,P=0.000).结论慢性压迫性脊髓症大鼠模型建立第4周,脊髓功能显著降低,同时BSCB结构破坏、通透性增加,MMP-9表达增强并与EBA抗原显著减少显著相关,提示BSCB破坏可能与MMP-9表达显著增高相关.
목적 관찰만성압박성척수증대서모형척수기질금속단백매-9(matrix metalloproteinase-9,MMP-9)표체화혈척수병장(blood spinal cord barrier,BSCB)적변화,탐토이자상관성.방법 장48지성년SD대서수궤분위가수술조화척수압박조,매조각24지;장흡수후가팽창취안지박판식입C5추판하경막외,건립만성압박성척수증모형.조모후제4주,량조분별통과BBB(Basso Beattie Bresnahan)평분화체감유발전위(somatosensory evoked potential,SEP)검측평개척수공능,매조각취12지행MMP-9、내피병장항원(endothelial barrier antigen,EBA)면역조화염색、검측이자재척수불동구역표체정황;기여12지행이문사람(evans blue,EB)관주검측BSCB통투성.통계학방법비교량조각관찰지표차이,병분석척수압박조MMP-9여EBA표체변화적상관성.결과 성공조모후제4주척수압박조BBB평분현저저우가수술조;상대우가수술조,척수압박조SEP검측잠복기현저연장、파폭현저강저.척수압박조백、회질MMP-9표체균현저고우가수술조,척수압박조회질EBA표체현저저우가수술조,백질EBA표체무현저변화,척수압박조EB함량현저고우가수술조;척수압박조MMP-9표체여EBA표체반정량결과진행배대양본상관성분석현시량자정현저상관(r=0.96,P=0.000).결론 만성압박성척수증대서모형건립제4주,척수공능현저강저,동시BSCB결구파배、통투성증가,MMP-9표체증강병여EBA항원현저감소현저상관,제시BSCB파배가능여MMP-9표체현저증고상관.
Objective To investigate the correlation between the expression of MMP-9 and the disruption of the blood spinal cord barrier (BSCB) in chronic cervical cord compressive myelopathy rat model.Methods 48 adult rats were randomly divided into group A (sham surgical group,n=24) and group B (spinal cord compressive group,n=24).A water-absorbing polymer sheet was implanted into the C5 epidural space on the posterolateral side to induce a chronic spinal cord compression model.BBB scores and SEP were collected 4 weeks after surgery.Expression of MMP-9 and endothelial barrier antigen (EBA) were detected immunohistochemically in different spinal cord areas of 12 rats in each group.The other 12 rats were performed with Evans blue (EB) perfusion to observe the permeability of BSCB.The results and the correlation between MMP-9 and EBA expression were analyzed.Results BBB score was lower in spinal cord compressive group than that of sham surgical group.Latency was delayed and amplitude was reduced significantly in spinal cord compressive group.Expression level of MMP-9 in spinal cord compressive group was significant higher than that in both grey matter and white matter in sham surgical group.In the contrary,EBA expression was obviously more in grey matter of sham surgical group than that in spinal cord compressive group,while it showed no significant change in white matter between the 2 groups.EB perfusion in sham surgical group was less than that in spinal cord compressive group.MMP-9 expression was correlated with EBA staining significantly.Conclusion Neurological function of spinal cord in the compression group was abnormal,with a marked decline 4 weeks after model made.The increased permeability and structural failure of BSCB in chronic spinal cord compression myelopathy,which was characterized and correlated with increment of MMP-9 expression and decrement of EBA expression concurrently.Disruption of BSCB may be correlated with distinct increasing expression of MMP-9.