环境科学
環境科學
배경과학
CHINESE JOURNAL OF ENVIRONMENTAL SCIENCE
2015年
5期
1749-1755
,共7页
梁贤%任勇翔%杨垒%赵思琪%夏志红
樑賢%任勇翔%楊壘%趙思琪%夏誌紅
량현%임용상%양루%조사기%하지홍
异养硝化%好氧反硝化%铜绿假单胞菌%脱氮%正交试验
異養硝化%好氧反硝化%銅綠假單胞菌%脫氮%正交試驗
이양초화%호양반초화%동록가단포균%탈담%정교시험
heterotrophic nitrification%aerobic denitrification%Pseudomonas aeruginosa%nitrogen removal%orthogonal experiment
针对传统自养硝化-厌氧反硝化工艺流程长、脱氮效率低的问题,从驯化成熟且具有高效同步硝化反硝化作用的 SBR反应器中筛得1株异养硝化菌 YL,经鉴定为铜绿假单胞菌(Pseudomonas aeruginosa),并通过单因子试验和正交试验对其异养硝化和好氧反硝化特性进行了研究.结果表明,菌株 YL 进行氨氧化作用的最适条件为:碳源为琥珀酸钠、 C/ N 为10、 pH 为7.0、温度为30℃、转速为160~200 r?min -1,此时氨氧化速率为5.05 mg?(g?h)-1,TOC 转化速率为45.95 mg?(g?h)-1,氨氮和 TOC 去除率分别为100%和90.8%;菌株 YL 还能够利用亚硝酸盐、硝酸盐和羟胺进行生长代谢,去除率分别为92.7%、93.6%和94.8%;影响菌株 YL 好氧反硝化性能最主要的因素为 C/ N,在最优条件(C/ N =10,t =30℃,r =200 r?min -1,pH =7)下,硝氮去除率为94.6%,总氮去除率76.3%.表明菌株 YL 能够独立快速高效地完成异养硝化和好氧反硝化脱氮过程.
針對傳統自養硝化-厭氧反硝化工藝流程長、脫氮效率低的問題,從馴化成熟且具有高效同步硝化反硝化作用的 SBR反應器中篩得1株異養硝化菌 YL,經鑒定為銅綠假單胞菌(Pseudomonas aeruginosa),併通過單因子試驗和正交試驗對其異養硝化和好氧反硝化特性進行瞭研究.結果錶明,菌株 YL 進行氨氧化作用的最適條件為:碳源為琥珀痠鈉、 C/ N 為10、 pH 為7.0、溫度為30℃、轉速為160~200 r?min -1,此時氨氧化速率為5.05 mg?(g?h)-1,TOC 轉化速率為45.95 mg?(g?h)-1,氨氮和 TOC 去除率分彆為100%和90.8%;菌株 YL 還能夠利用亞硝痠鹽、硝痠鹽和羥胺進行生長代謝,去除率分彆為92.7%、93.6%和94.8%;影響菌株 YL 好氧反硝化性能最主要的因素為 C/ N,在最優條件(C/ N =10,t =30℃,r =200 r?min -1,pH =7)下,硝氮去除率為94.6%,總氮去除率76.3%.錶明菌株 YL 能夠獨立快速高效地完成異養硝化和好氧反硝化脫氮過程.
침대전통자양초화-염양반초화공예류정장、탈담효솔저적문제,종순화성숙차구유고효동보초화반초화작용적 SBR반응기중사득1주이양초화균 YL,경감정위동록가단포균(Pseudomonas aeruginosa),병통과단인자시험화정교시험대기이양초화화호양반초화특성진행료연구.결과표명,균주 YL 진행안양화작용적최괄조건위:탄원위호박산납、 C/ N 위10、 pH 위7.0、온도위30℃、전속위160~200 r?min -1,차시안양화속솔위5.05 mg?(g?h)-1,TOC 전화속솔위45.95 mg?(g?h)-1,안담화 TOC 거제솔분별위100%화90.8%;균주 YL 환능구이용아초산염、초산염화간알진행생장대사,거제솔분별위92.7%、93.6%화94.8%;영향균주 YL 호양반초화성능최주요적인소위 C/ N,재최우조건(C/ N =10,t =30℃,r =200 r?min -1,pH =7)하,초담거제솔위94.6%,총담거제솔76.3%.표명균주 YL 능구독립쾌속고효지완성이양초화화호양반초화탈담과정.
Traditional process of autotrophic nitrification-anaerobic denitrification usually has problems of long procedure and low efficiency. To overcome these problems, a heterotrophic nitrification-aerobic denitrification bacterium YL was isolated from a domesticated mature SBR reactor with efficient simultaneous nitrification and denitrification ability, and was identified as Pseudomonas aeruginosa YL. Meanwhile, the characteristics of the nitrogen removal of strain YL was investigated through single - factor experiments and an orthogonal experiment. The results showed that the preferred conditions were: succinate as the carbon source, C/ N ratio of 10, pH of 7. 0, temperature of 30℃ , and the shaking speed of 160-200 r?min - 1 , while the removal rate of ammonia oxidation was 5. 05 mg?(g?h) - 1 , the transformation rate of TOC was 45. 95 mg?(g?h) - 1 , and the removal rates of nitrogen and TOC were 100% and 90. 8% , respectively. Nitrite, nitrate and hydroxylamine could also be metabolized by strain YL, and the removal rates were 92. 7% , 93. 6% and 94. 8% , respectively. The most important influencing factor on aerobic denitrification of strain YL was C/ N ratio. Under the optimal conditions (C/ N = 10, t = 30℃ , r = 200 r?min - 1 , pH = 7), the removal rates of nitrate and total nitrogen were 94. 6%and 76. 3% , respectively. Hence, strain YL could remove nitrogen by heterotrophic nitrification-aerobic denitrification independently, quickly, and effectively.