CAJ | 학술논문

黄连根茎和制剂具有抗菌等作用,广泛用于水产养殖,所造成水生态风险需要评估.试验设置总生物碱为0( CK),0.088(T1)、0.44(T2)和1.76 mg?L -1(T3)的黄连根茎浸提液(CRE)4种处理,研究了对斜生栅藻和蛋白核小球藻的毒理作用.结果表明,T1抑制绿藻生长,T2和 T3使绿藻生长和繁殖停止；它们均显著降低绿藻叶绿素和蛋白质含量,说明 CRE 抑制光合作用和蛋白质合成是绿藻生长繁殖速率降低和死亡的直接原因. CRE 使氢离子和胞内物质外流,导致藻液 pH 值显著降低和电导率提高.在 T1和 T2处理中,绿藻细胞 SOD 活性先升后降；在 T3处理中,SOD 活性显著降低.说明在 CRE 暴露初期,低中浓度的 CRE 诱导绿藻细胞产生抗性,随暴露时间增长或直接暴露在高浓度的 CRE 下,抗氧化酶系统被破坏.同样,随着 CRE 浓度增大,丙二醛含量增加,意味着绿藻细胞膜结构破坏,透性增加. CRE 总体上对蛋白核小球藻的危害作用大于斜生栅藻.在水产养殖中,滥用黄连根茎或制剂,以及大规模集约化种植黄连对水体初级生产力具有潜在的生态风险.
황련근경화제제구유항균등작용,엄범용우수산양식,소조성수생태풍험수요평고.시험설치총생물감위0( CK),0.088(T1)、0.44(T2)화1.76 mg?L -1(T3)적황련근경침제액(CRE)4충처리,연구료대사생책조화단백핵소구조적독리작용.결과표명,T1억제록조생장,T2화 T3사록조생장화번식정지；타문균현저강저록조협록소화단백질함량,설명 CRE 억제광합작용화단백질합성시록조생장번식속솔강저화사망적직접원인. CRE 사경리자화포내물질외류,도치조액 pH 치현저강저화전도솔제고.재 T1화 T2처리중,록조세포 SOD 활성선승후강；재 T3처리중,SOD 활성현저강저.설명재 CRE 폭로초기,저중농도적 CRE 유도록조세포산생항성,수폭로시간증장혹직접폭로재고농도적 CRE 하,항양화매계통피파배.동양,수착 CRE 농도증대,병이철함량증가,의미착록조세포막결구파배,투성증가. CRE 총체상대단백핵소구조적위해작용대우사생책조.재수산양식중,람용황련근경혹제제,이급대규모집약화충식황련대수체초급생산력구유잠재적생태풍험.
Coptis chinensis contains antiseptic alkaloids and thus its rhizomes and preparations are widely used for the treatment of fish diseases. In order to realize the risk of water ecosystems produced by this medical herb and preparations used in aquaculture, the present experiment was carried out to study the toxicity of Coptis chinensis rhizome extract (CRE) to Scenedesmus oblique and Chlorella pyrenoidosa grown in culture solution with 0. 00 (CK), 0. 088 (T1), 0. 44 (T2) and 1. 76 mg?L - 1 (T3) of CRE, respectively. The results show that low concentration of CRE (T1) inhibited the growth rate of the alga and high CRE (T2 and T3) ceased growth and reproductions. CRE also decreased the chlorophyll and proteins in alga cells, indicating the inhibition of photosynthesis and protein biosynthesis, which could be direct reasons for the low growth rate and death of green alga. The efflux of protons and substances from alga cells led to pH reduction and conductivity increment in culture solution with CRE. Furthermore, the activity of superoxide dismutase in alga increased at the beginning of CRE in T1 and T2 treatments but decreased as time prolonged which was in contrast to high CRE treatment. And the long exposure to low CRE treatment behaved otherwise. This suggests that the low concentration of CRE could induce the resistant reactions in alga at initial time but high CRE concentration or long exposure even at low CRE concentration could inhibit the enzyme synthesis. Similarly, malondialdehyde in alga increased as CRE concentrations increased in culture solutions, implying the damage and high permeability of cell membrane. In general,Chlorella pyrenoidosa was more sensitive to CRE. The abuse of rhizomes and preparations in aquaculture and intensive cultivation of Coptis chinensis plants in a large scale might produce ecological risks to primary productivity of water ecosystems.