CAJ | 학술논문

利用浮游植物荧光分类仪对暴露于不同浓度荔枝落叶浸出液下铜绿微囊藻的生长、最大光合作用效率(Fv / Fm )、实际光合作用效率(YⅡ)、光能利用效率(α)、最大相对电子传递速率(rETRmax)和光饱和系数(Ik)进行了为期15 d 的检测,研究浸出液对铜绿微囊藻生长和光合作用的影响.结果发现,浸泡5 d 的荔枝落叶浸出液可以抑制藻的生长,呈明显的浓度抑制型变化,随着时间的延长,抑制作用下滑.荧光参数 Fv / Fm、 YⅡ和α与浸出液浓度由负相关转为正相关或保持正相关关系,浸出液可能早期对藻光合作用发生胁迫,或通过提高光能利用效率来度过胁迫环境,rETRmax、 Ik 和叶绿素 a 浓度与浸出液浓度由负相关转为显著负相关关系.三维荧光图谱结果表明,第15 d 时,在投量为2.0 g?L -1时,色氨酸及酪氨酸荧光峰强度约为1.2 g?L -1投量情况下的1/3,同时腐殖酸的荧光峰强度减弱.进一步研究藻细胞生长的半抑制浓度 EC50值表明,与传统的农作物秸秆控藻比较,其 EC50值较低,可能荔枝落叶化感控藻效果较强,只需较低的浸出液浓度而能达到较好的控藻效果.
이용부유식물형광분류의대폭로우불동농도려지락협침출액하동록미낭조적생장、최대광합작용효솔(Fv / Fm )、실제광합작용효솔(YⅡ)、광능이용효솔(α)、최대상대전자전체속솔(rETRmax)화광포화계수(Ik)진행료위기15 d 적검측,연구침출액대동록미낭조생장화광합작용적영향.결과발현,침포5 d 적려지락협침출액가이억제조적생장,정명현적농도억제형변화,수착시간적연장,억제작용하활.형광삼수 Fv / Fm、 YⅡ화α여침출액농도유부상관전위정상관혹보지정상관관계,침출액가능조기대조광합작용발생협박,혹통과제고광능이용효솔래도과협박배경,rETRmax、 Ik 화협록소 a 농도여침출액농도유부상관전위현저부상관관계.삼유형광도보결과표명,제15 d 시,재투량위2.0 g?L -1시,색안산급락안산형광봉강도약위1.2 g?L -1투량정황하적1/3,동시부식산적형광봉강도감약.진일보연구조세포생장적반억제농도 EC50치표명,여전통적농작물갈간공조비교,기 EC50치교저,가능려지락협화감공조효과교강,지수교저적침출액농도이능체도교호적공조효과.
The growth and physiology of bloom-forming cyanobacterium Microcystis aeruginosa were determined by the pulse amplitude modulated fluorimetry when exposed to different concentrations of Litchi chinensis defoliation extract for 15 d. The growth, maximal efficiency ( Fv / Fm ), effective quantum yield ( YⅡ) of PSⅡ photochemistry, photosynthesis efficiency ( α), maximum electron transport rate (rETRmax) and light saturation coefficient (Ik) were used to evaluate the growth and photosynthesis in M. aeruginosa. It was found that the extract of L. chinensis defoliation stored for 5 days significantly inhibited the growth of M. aeruginosa in a concentration-dependent way. After a long time of exposure, stimulation effect disappeared gradually. Fv / Fm fluorescence parameters, YⅡ and alpha changed from negative correlation to positive correlation or kept positive correlation with the extract of L. chinensis defoliation, which might affect the photosynthesis of M. aeruginosa at early time or help the cyanobacterium to survive in the stress environment by improving the efficiency of light energy. Ik, rETRmax and the content of algal chlorophyll-a changed from negative to significant negative correlation with the extract. Three-dimensional fluorescence spectra showed that the peak intensities of tryptophan and tyrosine fluorescence were only about one third in 2. 0 g?L - 1 extract treatment when compared to the 1. 2 g?L - 1 extract treatment on day 15. At the same time, the peak intensity of humic acid fluorescence was weaker than that on day 1. Further study showed that the EC50 of algal growth was smaller than that of the traditional crops straw, which might achieve good effect to control the growth of algae with lower concentration of L. chinensis defoliation extract due to its strong allelopathy.