CAJ | 학술논문

目的观察共同性外斜视猫模型内直肌中神经生长因子(NGF)和生肌调节因子5(Myf5)表达变化及其与斜视时间的相关性.方法实验研究.选择处于视觉发育敏感期内幼猫(4～6周)18只,随机分为实验组和对照组;根据斜视时间实验组分为斜视1个月组、2个月组、3个月组,每组各3只;对照组随机分为对照1、2、3组(分别与斜视1个月、2个月、3个月组对应),每组各3只.实验组通过右眼外直肌缩短联合前徙制作外斜视模型,分别于术后1个月、2个月、3个月取实验组及相应对照组的右眼内直肌,固定、脱水、石蜡包埋、切片,免疫组织化学染色法检测内直肌NGF和Myf5平均光密度值,所得数据用小样本成组资料的t检验或t'检验、重复测量的方差分析及Pearson相关分析法进行统计.结果 ①实验组内直肌中NGF的平均光密度值低,斜视2个月组、3个月组与对应对照组内直肌中NGF的表达量进行比较,差异具有统计学意义(t=7.678、15.532,P＜0.01).②Myf5在实验组内直肌中的平均光密度值低,斜视2个月组、3个月组与其对照组内直肌Myf5的表达量差异具有统计学意义(t=5.819、7.029,P＜0.01).③外斜视猫模型内直肌中NGF、Myf5平均光密度值与斜视时间呈负相关(r=-0.984、-0.971,P＜0.05).结论 ①外科手术的方法可成功制作外斜视猫模型.②实验性外斜视猫内直肌中NGF和Myf5表达量的降低可能参与了斜视的发生.③实验性外斜视猫内直肌中NGF和Myf5的表达量随斜视病程的延长而降低,该变化可能与斜视病情的进展相关.
목적 관찰공동성외사시묘모형내직기중신경생장인자(NGF)화생기조절인자5(Myf5)표체변화급기여사시시간적상관성.방법 실험연구.선택처우시각발육민감기내유묘(4～6주)18지,수궤분위실험조화대조조;근거사시시간실험조분위사시1개월조、2개월조、3개월조,매조각3지;대조조수궤분위대조1、2、3조(분별여사시1개월、2개월、3개월조대응),매조각3지.실험조통과우안외직기축단연합전사제작외사시모형,분별우술후1개월、2개월、3개월취실험조급상응대조조적우안내직기,고정、탈수、석사포매、절편,면역조직화학염색법검측내직기NGF화Myf5평균광밀도치,소득수거용소양본성조자료적t검험혹t'검험、중복측량적방차분석급Pearson상관분석법진행통계.결과 ①실험조내직기중NGF적평균광밀도치저,사시2개월조、3개월조여대응대조조내직기중NGF적표체량진행비교,차이구유통계학의의(t=7.678、15.532,P＜0.01).②Myf5재실험조내직기중적평균광밀도치저,사시2개월조、3개월조여기대조조내직기Myf5적표체량차이구유통계학의의(t=5.819、7.029,P＜0.01).③외사시묘모형내직기중NGF、Myf5평균광밀도치여사시시간정부상관(r=-0.984、-0.971,P＜0.05).결론 ①외과수술적방법가성공제작외사시묘모형.②실험성외사시묘내직기중NGF화Myf5표체량적강저가능삼여료사시적발생.③실험성외사시묘내직기중NGF화Myf5적표체량수사시병정적연장이강저,해변화가능여사시병정적진전상관.
Objective To observe the changes in the expression of nerve growth factor (NGF) and myocyte differentiation factor 5(Myf5) in the medial rectus muscle of a cat model with strabismus.Methods Eighteen cats that were in the 4-6 weeks sensitive period were selected for surgery to create an exotropia model.All animals were randomly divided into experimental and control groups.Medial rectus muscles from both the experimental and control groups were collected at 1 month,2 months,and 3 months after strabismus surgery.These samples were prepared for histologic examination by fixation,paraffin embedding and slicing.Immunohistochemistry staining was used to detect NGF and Myf5 expression and an optical density analysis was used to measure the average optical density.Data were analyzed using t test or t' test,repeat measured ANOVA and Pearson correlation test.Results ①There were significant differences in NGF expression in the medial rectus muscles of the experimental and control groups between 2 and 3 months (t=7.678,15.532,P＜0.01).②There were also significant differences in the expression of Myf5 in the medial rectus muscles of the experimental and control groups between 2 and 3 months (t=5.819,7.029,P＜0.01).③There was a negative relationship between NGF and Myf5 expression and the development of strabismus (r=-0.984,--0.971,P＜0.05).Conclusion ①he surgical method can successfully produce a concomitant exotropia cat model.②The reduced expression of NGF and Myf5 in the medial rectus muscle in the experimental strabismus cat model may be involved in the occurrence of strabismus.③NGF and Myf5 expression in the medial rectus muscle are reduced with the duration of the extension,which may be related to the progress of a strabismus condition.