中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2015年
2期
107-109
,共3页
张尧%杨柠泽%王志军%邱伟%刘容嘉%苏晓玮%贾华锋
張堯%楊檸澤%王誌軍%邱偉%劉容嘉%囌曉瑋%賈華鋒
장요%양저택%왕지군%구위%류용가%소효위%가화봉
三维%可视化%眶颊联合%模型
三維%可視化%眶頰聯閤%模型
삼유%가시화%광협연합%모형
Three-dimensional%Visualization%Orbital buccal joint%Model
目的 通过对眶颊联合连续石蜡切片染色后三维可视化,探讨重建眶颊联合三维模型的方法,初步建立眶颊联合的三维可视化虚拟模型,为临床提供解剖学依据及临床教学提供数字化参考模型.方法 切取1例成人尸头的眶颊联合全层组织,大小为30 mm×20 mm×20 mm.用石蜡包埋,切片,切片厚度为15 μm,获得切片200张,用苏术精-伊红(HE)染色及Masson染色,用索尼相机拍照,利用Adobe photoshop CS5.1图像处理软件进行图像配准,用3D-doctor软件将图像分割及赋予不同颜色,完成三维模型建立.结果 (1)从组织学上证实了眶筋膜增厚区(LOT)的存在,重建了LOT的模型.(2)显示了LOT组织学特性:Masson染色显示为红蓝相间的组织,根据染色结果推断LOT为弹力纤维与胶原纤维相互交织的筋膜组织.(3)LOT呈底边朝向眼眶的三角形,底边长26 mm,高31 mm,厚0.8 mm,面积约为4.03 cm2.(4)在三维模型中可见皮肤层、眼轮匝肌、眼轮匝肌下脂肪垫(SOOF)、眶脂、骨膜、眼轮匝肌支持韧带及LOT.(5)通过对眶颊联合的三维模型可显示其相邻关系,可旋转任意角度显示.结论 初步建立了眶颊联合的三维模型,证实了眶颊联合的组织学特性及重建软组织三维局部内部细微结构的可行性.
目的 通過對眶頰聯閤連續石蠟切片染色後三維可視化,探討重建眶頰聯閤三維模型的方法,初步建立眶頰聯閤的三維可視化虛擬模型,為臨床提供解剖學依據及臨床教學提供數字化參攷模型.方法 切取1例成人尸頭的眶頰聯閤全層組織,大小為30 mm×20 mm×20 mm.用石蠟包埋,切片,切片厚度為15 μm,穫得切片200張,用囌術精-伊紅(HE)染色及Masson染色,用索尼相機拍照,利用Adobe photoshop CS5.1圖像處理軟件進行圖像配準,用3D-doctor軟件將圖像分割及賦予不同顏色,完成三維模型建立.結果 (1)從組織學上證實瞭眶觔膜增厚區(LOT)的存在,重建瞭LOT的模型.(2)顯示瞭LOT組織學特性:Masson染色顯示為紅藍相間的組織,根據染色結果推斷LOT為彈力纖維與膠原纖維相互交織的觔膜組織.(3)LOT呈底邊朝嚮眼眶的三角形,底邊長26 mm,高31 mm,厚0.8 mm,麵積約為4.03 cm2.(4)在三維模型中可見皮膚層、眼輪匝肌、眼輪匝肌下脂肪墊(SOOF)、眶脂、骨膜、眼輪匝肌支持韌帶及LOT.(5)通過對眶頰聯閤的三維模型可顯示其相鄰關繫,可鏇轉任意角度顯示.結論 初步建立瞭眶頰聯閤的三維模型,證實瞭眶頰聯閤的組織學特性及重建軟組織三維跼部內部細微結構的可行性.
목적 통과대광협연합련속석사절편염색후삼유가시화,탐토중건광협연합삼유모형적방법,초보건립광협연합적삼유가시화허의모형,위림상제공해부학의거급림상교학제공수자화삼고모형.방법 절취1례성인시두적광협연합전층조직,대소위30 mm×20 mm×20 mm.용석사포매,절편,절편후도위15 μm,획득절편200장,용소술정-이홍(HE)염색급Masson염색,용색니상궤박조,이용Adobe photoshop CS5.1도상처리연건진행도상배준,용3D-doctor연건장도상분할급부여불동안색,완성삼유모형건립.결과 (1)종조직학상증실료광근막증후구(LOT)적존재,중건료LOT적모형.(2)현시료LOT조직학특성:Masson염색현시위홍람상간적조직,근거염색결과추단LOT위탄력섬유여효원섬유상호교직적근막조직.(3)LOT정저변조향안광적삼각형,저변장26 mm,고31 mm,후0.8 mm,면적약위4.03 cm2.(4)재삼유모형중가견피부층、안륜잡기、안륜잡기하지방점(SOOF)、광지、골막、안륜잡기지지인대급LOT.(5)통과대광협연합적삼유모형가현시기상린관계,가선전임의각도현시.결론 초보건립료광협연합적삼유모형,증실료광협연합적조직학특성급중건연조직삼유국부내부세미결구적가행성.
Objective To explore the methodology of reconstruction of a three-dimensional model of lid/cheek junction through continuous paraffin sections and stained lid/cheek junction three-dimensional visualization,and to further explore the feasibility and reliability to provide anatomical basis for clinical teaching of plastic and reconstructive surgery.Methods The full size of lid/cheek junction was cut from the specimens,the size of 25 mm× 15 mm× 10 mm,and then embedded in paraffin,and sectioned in thickness of 15 μm for 200 slices;the experimental HE staining and Masson staining were conducted,Sony camera photos using Adobe Photoshop CS 5.1 image processing software for image registration and 3D-Docter software image segmentation were used to give different colors for establishment of complete three-dimensional model.Results Histologically the LOT of the fascia area,the existence of reconstruction and the LOT model were confirmed.It showed the histological characteristics:the Masson stain displayed red,blue and white tissues in color;fascia tissue staining infered intertexture of elastic fibers and collagen fibers in LOT.LOT was the bottom edge of the triangle toward the orbital base with length of 26 mm,31 mm in high,0.8 mm in thick,and area of approximately 4.03 cm2 in size.Layers of skin,orbicularis oculi muscle,orbicularis retaining ligament,middle temporal fascia,periosteum and LOT were visible in the 3D model.By the three-dimensional model of lid/cheek junction,adjacent relationship could be rotated to any angle.Conclusions This initial establishment of a three-dimensional model of the lid/cheek junction confirms that the histological characteristics of lid/cheek junction and the feasibility of the fine structure of soft tissue within the three-dimensional model can be used as a new method for further research.
