中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2015年
2期
103-106
,共4页
三氧化矿物聚合物%牙髓%干细胞%恒牙
三氧化礦物聚閤物%牙髓%榦細胞%恆牙
삼양화광물취합물%아수%간세포%항아
Mineral trioxide aggregate%Dental pulp%Stem cells%Permanent teeth
目的 探讨不同浓度三氧化矿物聚合物(MTA)对年轻恒牙牙髓干细胞(DPSCs)体外增殖分化的影响.方法 从年轻恒牙中分离出牙髓组织,用组织贴块法培养DPSCs,并通过检测干细胞表面标志物STRO-1进行鉴定.制备浓度为0.02、0.20、2.00、20 g/L的MTA浸提液.采用四甲基偶氮噻唑蓝比色法(MTT)检测不同浓度MTA对DPSCs存活率及增殖的影响;加入适宜浓度MTA培养4周后,茜素红染色检测矿化结节形成情况;荧光定量聚合酶链反应(PCR)分别测定适宜浓度MTA培养12、24、36、48 h后碱性磷酸酶(ALP)、骨涎蛋白、骨钙素、牙本质涎蛋白(DSP)mRNA的表达.结果 免疫荧光显示干细胞表面标志物表达呈阳性.浓度为0.20 g/L的MTA促进DPSCs增殖的活力较强,培养4周后茜素红染色可见有矿化结节形成.作用于DPSCs不同时间后,骨钙素和DSP mRNA的表达水平呈时间依赖性,在48 h内随着时间的增长而上升.而ALP和BSP mRNA的表达水平则是先上升后下降.结论 0.02、0.20、2.00 g/L浓度的MTA能有效地促进年轻恒牙DPSCs增殖,且0.20 g/L MTA能有效地诱导DPSCs向成牙本质细胞分化.
目的 探討不同濃度三氧化礦物聚閤物(MTA)對年輕恆牙牙髓榦細胞(DPSCs)體外增殖分化的影響.方法 從年輕恆牙中分離齣牙髓組織,用組織貼塊法培養DPSCs,併通過檢測榦細胞錶麵標誌物STRO-1進行鑒定.製備濃度為0.02、0.20、2.00、20 g/L的MTA浸提液.採用四甲基偶氮噻唑藍比色法(MTT)檢測不同濃度MTA對DPSCs存活率及增殖的影響;加入適宜濃度MTA培養4週後,茜素紅染色檢測礦化結節形成情況;熒光定量聚閤酶鏈反應(PCR)分彆測定適宜濃度MTA培養12、24、36、48 h後堿性燐痠酶(ALP)、骨涎蛋白、骨鈣素、牙本質涎蛋白(DSP)mRNA的錶達.結果 免疫熒光顯示榦細胞錶麵標誌物錶達呈暘性.濃度為0.20 g/L的MTA促進DPSCs增殖的活力較彊,培養4週後茜素紅染色可見有礦化結節形成.作用于DPSCs不同時間後,骨鈣素和DSP mRNA的錶達水平呈時間依賴性,在48 h內隨著時間的增長而上升.而ALP和BSP mRNA的錶達水平則是先上升後下降.結論 0.02、0.20、2.00 g/L濃度的MTA能有效地促進年輕恆牙DPSCs增殖,且0.20 g/L MTA能有效地誘導DPSCs嚮成牙本質細胞分化.
목적 탐토불동농도삼양화광물취합물(MTA)대년경항아아수간세포(DPSCs)체외증식분화적영향.방법 종년경항아중분리출아수조직,용조직첩괴법배양DPSCs,병통과검측간세포표면표지물STRO-1진행감정.제비농도위0.02、0.20、2.00、20 g/L적MTA침제액.채용사갑기우담새서람비색법(MTT)검측불동농도MTA대DPSCs존활솔급증식적영향;가입괄의농도MTA배양4주후,천소홍염색검측광화결절형성정황;형광정량취합매련반응(PCR)분별측정괄의농도MTA배양12、24、36、48 h후감성린산매(ALP)、골연단백、골개소、아본질연단백(DSP)mRNA적표체.결과 면역형광현시간세포표면표지물표체정양성.농도위0.20 g/L적MTA촉진DPSCs증식적활력교강,배양4주후천소홍염색가견유광화결절형성.작용우DPSCs불동시간후,골개소화DSP mRNA적표체수평정시간의뢰성,재48 h내수착시간적증장이상승.이ALP화BSP mRNA적표체수평칙시선상승후하강.결론 0.02、0.20、2.00 g/L농도적MTA능유효지촉진년경항아DPSCs증식,차0.20 g/L MTA능유효지유도DPSCs향성아본질세포분화.
Objective To investigate the effects of the different concentration of mineral trioxide aggregate (MTA) on the proliferation and differentiation of dental pulp stem cells (DPSCs) from the young permanent teeth.Methods DPSCs were isolated from the young permanent teeth and cultured by tissue explant method.The expression of STRO-1 was detected by using immunofluorescence technology.DPSCs were cultured with different concentrations of MTA (0.02,0.20,2.00,20.00 g/L).Cell proliferation was detected by MTT array.Cells were cultured in the appropriate concentration of MTA for 4 weeks,and then stained by Alizarin red to detect their mineralized nodule formation capacity.The cells were cultured with the appropriate concentration of MTA and collected after 12,24,36,48 h.The mRNA expression of ALP,BSP,OC and DSP after the treatment of MTA were detected by quantitative PCR.Results DPSCs were positive for STRO-1.The capacity of 0.20 g/L MTA promoting the proliferation of DPSCs was stronger than other concentrations.After 4 weeks,the mineralized nodules of DPSCs were observed after alizarin red staining.The PCR showed that with increasing induction time,the expression levels of DSP and OC were up-regulated.But that of ALP and BSP was increased first and then decreased.Conclusions In this study,MTA can promote the proliferation of DPSCs at 0.02,0.20,2.00 g/L concentration.It can induce odontoblast differentiation effectively by 0.20 g/L MTA.
