中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2015年
5期
312-316
,共5页
陈绍椿%尹跃平%戴秀芹%郑和平%顾伟鸣%郑钟洁%吴兴中%曹文苓%胡丽华%朱邦勇%孙厚华%陈祥生
陳紹椿%尹躍平%戴秀芹%鄭和平%顧偉鳴%鄭鐘潔%吳興中%曹文苓%鬍麗華%硃邦勇%孫厚華%陳祥生
진소춘%윤약평%대수근%정화평%고위명%정종길%오흥중%조문령%호려화%주방용%손후화%진상생
奈瑟球菌,淋病%β内酰胺酶类%流行病学%blaTEM-135突变体%NG-MAST
奈瑟毬菌,淋病%β內酰胺酶類%流行病學%blaTEM-135突變體%NG-MAST
내슬구균,임병%β내선알매류%류행병학%blaTEM-135돌변체%NG-MAST
Neisseria gonorrhoeae%beta-Lactamases%Epidemiology%blaTEM-135 Gene variant%Neisseria gonorrhoeae multi-antigen sequence typing
目的:了解中国不同地区淋球菌耐药监测点产青霉素酶淋球菌(PPNG)的比例及 blaTEM-135突变体在 PPNG 中的分布,比较 PPNG 及 blaTEM-135突变体淋球菌多抗原测序分型(NG-MAST)的型别分布,了解不同地区 PPNG blaTEM-135突变体的差异与联系。方法2012年在江苏、上海、浙江、天津、广东、广西6个淋球菌耐药监测点共收集572株淋球菌,经过分离纯化及鉴定后,采用头孢噻吩纸片法测定 PPNG;菌株培养后利用试剂盒提取 DNA,通过错配扩增突变分析 PCR(MAMA PCR)鉴定 blaTEM-135突变体,采用 NG-MAST 进行分型研究。结果572株淋球菌中 PPNG 总阳性率为38.1%(218/572),PPNG 中相应 blaTEM-135突变体的总比例为52.3%(114/218)。监测点中 PPNG 阳性率从高至低分别为:浙江(45/87,51.7%)、上海(36/79,45.6%)、广东(78/205,38.0%)、广西(12/32,37.5%)、江苏(24/77,31.2%)、天津(23/92,25%);PPNG 中相应 blaTEM-135突变体的阳性率从高至低分别为:浙江(31/45,68.9%)、江苏(14/24,58.3%)、广东(39/78,50.0%)、上海(17/36,47.2%)、天津(9/23,39.1%)、广西(4/12,33.3%)。 NG-MAST 分型研究显示,blaTEM-135突变体中流行菌株型别有 ST2318、ST1768、ST1866、ST1053、ST8726等,其中 ST1768、ST1053和 ST8726与 blaTEM-135突变体有较强的对应关系。天津 PPNG 菌株及 blaTEM-135突变体 ST 分布与其他各监测点有显著差异,江浙沪地区菌株 ST 有一定联系。结论中国淋球菌耐药监测点 PPNG 及相应 blaTEM-135突变体阳性率处于较高水平,不同地区间 PPNG 及相应 blaTEM-135突变体阳性菌株 ST 型别分布差异有统计学意义。
目的:瞭解中國不同地區淋毬菌耐藥鑑測點產青黴素酶淋毬菌(PPNG)的比例及 blaTEM-135突變體在 PPNG 中的分佈,比較 PPNG 及 blaTEM-135突變體淋毬菌多抗原測序分型(NG-MAST)的型彆分佈,瞭解不同地區 PPNG blaTEM-135突變體的差異與聯繫。方法2012年在江囌、上海、浙江、天津、廣東、廣西6箇淋毬菌耐藥鑑測點共收集572株淋毬菌,經過分離純化及鑒定後,採用頭孢噻吩紙片法測定 PPNG;菌株培養後利用試劑盒提取 DNA,通過錯配擴增突變分析 PCR(MAMA PCR)鑒定 blaTEM-135突變體,採用 NG-MAST 進行分型研究。結果572株淋毬菌中 PPNG 總暘性率為38.1%(218/572),PPNG 中相應 blaTEM-135突變體的總比例為52.3%(114/218)。鑑測點中 PPNG 暘性率從高至低分彆為:浙江(45/87,51.7%)、上海(36/79,45.6%)、廣東(78/205,38.0%)、廣西(12/32,37.5%)、江囌(24/77,31.2%)、天津(23/92,25%);PPNG 中相應 blaTEM-135突變體的暘性率從高至低分彆為:浙江(31/45,68.9%)、江囌(14/24,58.3%)、廣東(39/78,50.0%)、上海(17/36,47.2%)、天津(9/23,39.1%)、廣西(4/12,33.3%)。 NG-MAST 分型研究顯示,blaTEM-135突變體中流行菌株型彆有 ST2318、ST1768、ST1866、ST1053、ST8726等,其中 ST1768、ST1053和 ST8726與 blaTEM-135突變體有較彊的對應關繫。天津 PPNG 菌株及 blaTEM-135突變體 ST 分佈與其他各鑑測點有顯著差異,江浙滬地區菌株 ST 有一定聯繫。結論中國淋毬菌耐藥鑑測點 PPNG 及相應 blaTEM-135突變體暘性率處于較高水平,不同地區間 PPNG 及相應 blaTEM-135突變體暘性菌株 ST 型彆分佈差異有統計學意義。
목적:료해중국불동지구림구균내약감측점산청매소매림구균(PPNG)적비례급 blaTEM-135돌변체재 PPNG 중적분포,비교 PPNG 급 blaTEM-135돌변체림구균다항원측서분형(NG-MAST)적형별분포,료해불동지구 PPNG blaTEM-135돌변체적차이여련계。방법2012년재강소、상해、절강、천진、엄동、엄서6개림구균내약감측점공수집572주림구균,경과분리순화급감정후,채용두포새분지편법측정 PPNG;균주배양후이용시제합제취 DNA,통과착배확증돌변분석 PCR(MAMA PCR)감정 blaTEM-135돌변체,채용 NG-MAST 진행분형연구。결과572주림구균중 PPNG 총양성솔위38.1%(218/572),PPNG 중상응 blaTEM-135돌변체적총비례위52.3%(114/218)。감측점중 PPNG 양성솔종고지저분별위:절강(45/87,51.7%)、상해(36/79,45.6%)、엄동(78/205,38.0%)、엄서(12/32,37.5%)、강소(24/77,31.2%)、천진(23/92,25%);PPNG 중상응 blaTEM-135돌변체적양성솔종고지저분별위:절강(31/45,68.9%)、강소(14/24,58.3%)、엄동(39/78,50.0%)、상해(17/36,47.2%)、천진(9/23,39.1%)、엄서(4/12,33.3%)。 NG-MAST 분형연구현시,blaTEM-135돌변체중류행균주형별유 ST2318、ST1768、ST1866、ST1053、ST8726등,기중 ST1768、ST1053화 ST8726여 blaTEM-135돌변체유교강적대응관계。천진 PPNG 균주급 blaTEM-135돌변체 ST 분포여기타각감측점유현저차이,강절호지구균주 ST 유일정련계。결론중국림구균내약감측점 PPNG 급상응 blaTEM-135돌변체양성솔처우교고수평,불동지구간 PPNG 급상응 blaTEM-135돌변체양성균주 ST 형별분포차이유통계학의의。
Objective To determine the prevalence of penicillinase-producing Neisseria gonorrhoeae(PPNG) and the distribution of blaTEM-135 gene variants in PPNG at several gonococcal antimicrobial surveillance sites in China, to compare N. gonorrhoeae multi-antigen sequence typing(NG-MAST)types of PPNG and its blaTEM-135 gene variants, and to assess the difference and association in NG-MAST types of blaTEM-135 gene variants among different regions. Methods A total of 572 N. gonorrhoeae isolates were collected at 6 gonococcal antimicrobial surveillance sites from Jiangsu, Shanghai, Zhejiang, Tianjin, Guangdong and Guangxi in 2012. After isolation, purification, and identification, cefalotin paper discs were used for detection of PPNG. DNA was extracted by QIAxtractor DX kits after cultivation of the PPNG strains. Then, mismatch amplification mutation assay (MAMA) PCR was performed to identify blaTEM-135 variants, and NG-MAST analysis to determine N. gonorrhoeae genotypes. Results Among the 572 N. gonorrhoeae strains, 38.1%(218/572) were identified as PPNG, and of the PPNG strains, 52.3% (114/218) were blaTEM-135 variants. The detection rate of PPNG at these surveillance sites from high to low was as follows: 51.7% (45/87, Zhejiang), 45.6%(36/79, Shanghai), 38.0% (78/205, Guangdong), 37.5% (12/32, Guangxi), 31.2% (24/77, Jiangsu) and 25.0%(23/92, Tianjin), and that of blaTEM-135 variants was as follows: 68.9%(31/45, Zhejiang), 58.3%(14/24, Jiangsu), 50.0%(39/78, Guangdong), 47.2%(17/36, Shanghai), 39.1%(9/23, Tianjin)and 33.3%(4/12, Guangxi). NG-MAST analysis showed that the ST2318, ST1768, ST1866, ST1053 and ST8726 types predominated among these bla TEM-135 variants, and a strong correlation was found between blaTEM-135 variants and some NG-MAST types, such as ST1768, ST1053 and ST8726 types. The distribution of NG-MAST types was significantly different between the surveillance site in Tianjin (in the Northern part of China) and the other sites (in the Southern part of China), but highly similar among the surveillance sites in Jiangsu, Zhejiang and Shanghai regions. Conclusions There is a high prevalence of PPNG and its blaTEM-135 variants at several gonococcal antimicrobial surveillance sites in China, with significant differences in NG-MAST genotype distribution of PPNG and its blaTEM-135 variants among different regions.