中西医结合肝病杂志
中西醫結閤肝病雜誌
중서의결합간병잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE ON LIVER DISEASES
2015年
2期
92-94
,共3页
陈高峰%平键%高月求%金树根
陳高峰%平鍵%高月求%金樹根
진고봉%평건%고월구%금수근
二甲基亚硝胺%小鼠%年龄%性别%急性肝损伤%出血性坏死%腹水
二甲基亞硝胺%小鼠%年齡%性彆%急性肝損傷%齣血性壞死%腹水
이갑기아초알%소서%년령%성별%급성간손상%출혈성배사%복수
dimethylnitrosamine%mice%age%gender%acute liver injury%hemorrhagic liver necrosis%ascites
目的：研究年龄与性别对二甲基亚硝胺（DMN）中毒所致小鼠急性肝损伤的影响。方法：采用一次性 DMN 17．5μl／kg腹腔内注射染毒致4、8、12周龄雌雄性小鼠急性肝损伤，通过肝功能血清生化指标[丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、直接胆红素（DBil）、血清白蛋白（Alb）和总胆固醇（TC）]检测和肝组织 HE 染色病理学检查，观察不同性别和年龄小鼠急性肝损伤变化特征。结果：DMN 染毒48h 后致4周龄小鼠血清 ALT、AST 上升和肝内出血性坏死，以雌性小鼠病变更为明显，部分雌性小鼠出现腹水。8周龄和12周龄小鼠血清 ALT、AST 上升和肝内出血性坏死更为明显，以雌性小鼠病变极为严重，12周龄小鼠肝呈花斑状，雌性小鼠全部出现腹水并出现死亡，8周龄和12周龄小鼠各有2只雄性小鼠出现腹水。结论： DMN 中毒所致小鼠急性肝损伤与年龄和性别密切相关，以雌性、成年小鼠更加明显，表现为肝内出血性坏死和腹水为特征。
目的：研究年齡與性彆對二甲基亞硝胺（DMN）中毒所緻小鼠急性肝損傷的影響。方法：採用一次性 DMN 17．5μl／kg腹腔內註射染毒緻4、8、12週齡雌雄性小鼠急性肝損傷，通過肝功能血清生化指標[丙氨痠氨基轉移酶（ALT）、天門鼕氨痠氨基轉移酶（AST）、直接膽紅素（DBil）、血清白蛋白（Alb）和總膽固醇（TC）]檢測和肝組織 HE 染色病理學檢查，觀察不同性彆和年齡小鼠急性肝損傷變化特徵。結果：DMN 染毒48h 後緻4週齡小鼠血清 ALT、AST 上升和肝內齣血性壞死，以雌性小鼠病變更為明顯，部分雌性小鼠齣現腹水。8週齡和12週齡小鼠血清 ALT、AST 上升和肝內齣血性壞死更為明顯，以雌性小鼠病變極為嚴重，12週齡小鼠肝呈花斑狀，雌性小鼠全部齣現腹水併齣現死亡，8週齡和12週齡小鼠各有2隻雄性小鼠齣現腹水。結論： DMN 中毒所緻小鼠急性肝損傷與年齡和性彆密切相關，以雌性、成年小鼠更加明顯，錶現為肝內齣血性壞死和腹水為特徵。
목적：연구년령여성별대이갑기아초알（DMN）중독소치소서급성간손상적영향。방법：채용일차성 DMN 17．5μl／kg복강내주사염독치4、8、12주령자웅성소서급성간손상，통과간공능혈청생화지표[병안산안기전이매（ALT）、천문동안산안기전이매（AST）、직접담홍소（DBil）、혈청백단백（Alb）화총담고순（TC）]검측화간조직 HE 염색병이학검사，관찰불동성별화년령소서급성간손상변화특정。결과：DMN 염독48h 후치4주령소서혈청 ALT、AST 상승화간내출혈성배사，이자성소서병변경위명현，부분자성소서출현복수。8주령화12주령소서혈청 ALT、AST 상승화간내출혈성배사경위명현，이자성소서병변겁위엄중，12주령소서간정화반상，자성소서전부출현복수병출현사망，8주령화12주령소서각유2지웅성소서출현복수。결론： DMN 중독소치소서급성간손상여년령화성별밀절상관，이자성、성년소서경가명현，표현위간내출혈성배사화복수위특정。
Objective:To study the effects of age and gender on dimethylnitrosamine (DMN) -induced acute toxic liver injury in mice. Methods: Male and female mice of 4,8,12 weeks old were intraperitoneally injected with DMN with dosage of 17.5μl /kg once a time to in-duce acute liver injury, after 48 hours serum biochemical parameters of liver function [including albumin(Alb), alanine aminotransferase (ALT) ,aspartate aminotransferase (AST), direct bilirubin (DBil),total cholesterol(TC)] and HE staining of liver tissue were detected to observe the characteristic changes of acute liver injury with different age and gender.Results:Forty-eight hours after the intraperitoneal injec-tion of DMN with dosage of 20mg/kg once a time caused increase of serum ALT, AST and DBil, decrease of serum of Alb and TC,hemor-rhagic necrosis of the liver in mice of 4 weeks old.Those changes were significantly evident in the female mice, some female mice had asci-tes.With the increase of age, the above changes became more evident, all female aged mice of 8 and 12 weeks old had serious ascites, and some male aged mice had ascites, deaths only occurred in female aged mice of 8 and 12 weeks old.Conclusion:The acute liver injury in mice induced by DMN is closely related with age and gender, the injury is more pronounced in female aged mice,which manifestations are hemor-rhagic liver necrosis and ascites.
