CAJ | 학술논문

目的：研究利用高脂联合高糖饲养C57 BL/6小鼠建立糖尿病小鼠角膜病变模型。 　　方法：建立利用高脂联合高糖饲养C57 BL/6小鼠的糖尿病模型,在成模2~12 mo分别行1%虎红染色检查角膜上皮完整性；角膜上皮刮除后,荧光素钠染色观察比较角膜上皮的愈合速度；病理检测角膜的组织形态和结构的变化。 　　结果：高脂联合高糖饲养C57 BL/6小鼠2 mo左右逐渐表现出糖尿病多饮、多食、多尿、体质量下降等典型症状,其血糖稳定维持在较高水平(≥18mmol/L),体质量较正常小鼠明显偏低。虎红染色发现,成模2 mo的小鼠角膜即已出现点状着色,且随着病程的延长,染色面积和程度逐渐加重,到成模12 mo几乎呈全角膜着色。在上皮愈合速度方面,刮除角膜上皮后,成模2 mo的小鼠角膜上皮在40 h即完全愈合(与正常小鼠相似),成模3 mo小鼠上皮愈合时间为120h,成模4,6,12mo上皮完全愈合的时间为144 h左右,其中成模12 mo的小鼠在96~120 h上皮缺损的范围再次扩大,后逐渐缩小愈合,呈现反复现象。 　　结论：高脂联合高糖饲养诱导的糖尿病小鼠角膜,表现出角膜上皮损害,角膜上皮损伤后延迟愈合等症状,说明其可以作为研究糖尿病角膜病变的动物模型。
목적：연구이용고지연합고당사양C57 BL/6소서건립당뇨병소서각막병변모형。 　　방법：건립이용고지연합고당사양C57 BL/6소서적당뇨병모형,재성모2~12 mo분별행1%호홍염색검사각막상피완정성；각막상피괄제후,형광소납염색관찰비교각막상피적유합속도；병리검측각막적조직형태화결구적변화。 　　결과：고지연합고당사양C57 BL/6소서2 mo좌우축점표현출당뇨병다음、다식、다뇨、체질량하강등전형증상,기혈당은정유지재교고수평(≥18mmol/L),체질량교정상소서명현편저。호홍염색발현,성모2 mo적소서각막즉이출현점상착색,차수착병정적연장,염색면적화정도축점가중,도성모12 mo궤호정전각막착색。재상피유합속도방면,괄제각막상피후,성모2 mo적소서각막상피재40 h즉완전유합(여정상소서상사),성모3 mo소서상피유합시간위120h,성모4,6,12mo상피완전유합적시간위144 h좌우,기중성모12 mo적소서재96~120 h상피결손적범위재차확대,후축점축소유합,정현반복현상。 　　결론：고지연합고당사양유도적당뇨병소서각막,표현출각막상피손해,각막상피손상후연지유합등증상,설명기가이작위연구당뇨병각막병변적동물모형。
AIM: To discuss the establishment of immediate diabetic keratopathy animal model of C57BL/6 mouse induced by ahigh-fat and high-glucose diet. METHODS: Diabetes mellitus was induced by a high-fat and high-glucose diet in C57BL/6 mouse. 1% rose bengal was stained on the cornea to examine the integrality of the corneal epithelium at 2 ~ 12mo after completion of the model. Corneal epithelial wound healing was observed using a vivo epithelial debridement model which was dyed by sodium fluorescein. Corneal morphology histology was examined by pathological methods. RESULTS: The high-fat and high-glucose diet C57BL/6 mouse in 2mo had showed general symptoms of diabetes: polydipsia, polyphagia, polyuria, weight loss etc. The model had a steady-state high glucose (≥18mmol/L), also the weight was lower compared with normal control mouse. 1% rose bengal corneal staining had dot coloring at 2mo after completion of the model, the stained area and extent were gradually increased with the extension of the duration of diabetes, almost all the cornea was stained at 12mo after completion of the model. With the passage of time into a mold, the cornea epithelial healing time become longer: 2mo was about 40h;3mo was about 120h; 4, 6, 12mo was about 144h;the coloboma were gradually increased at 12mo after completion of the model, then the area was reduced gradually until complete healing, the time was 96~120h, showed repeating phenomenon. CONCLUSION: The mouse were induced by high-fat and high-glucose diet can be used as animal models of diabetic keratopathy: the damage of epithelium for corneal and delay healing on epithelium and other symptoms.