中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2015年
4期
262-265
,共4页
刘影%陈晓玲%丁宁宁%徐文祥%杨虹%陈学琛
劉影%陳曉玲%丁寧寧%徐文祥%楊虹%陳學琛
류영%진효령%정저저%서문상%양홍%진학침
间皮瘤%基因,p16%原位杂交,荧光%免疫组织化学
間皮瘤%基因,p16%原位雜交,熒光%免疫組織化學
간피류%기인,p16%원위잡교,형광%면역조직화학
Mesothelioma%Genes,p16%In situ hybridization,fluorescence%Immunohistochemistry
目的:探讨p16基因缺失及p16蛋白的表达情况在鉴别恶性间皮瘤与良性反应性间皮细胞增生中的作用。方法采用荧光原位杂交( FISH)和免疫组织化学分别检测55例间皮瘤及30例良性反应性间皮细胞增生中p16基因和蛋白的表达情况,对两种方法的检测结果进行对比分析。结果在55例恶性间皮瘤组织学标本中,p16基因缺失率为81.8%(45/55),高于良性间皮细胞增生者(3.3%,1/30),差异有统计学意义( P <0.05);恶性间皮瘤组织学标本中 p16蛋白表达率为23.6%,低于良性间皮细胞增生者(76.7%);FISH检测恶性间皮瘤组织中p16基因缺失敏感度及特异度均高于免疫组织化学方法检测p16蛋白者。结论恶性间皮瘤中p16基因缺失,p16蛋白阴性表达。 FISH检测恶性间皮瘤组织中p16基因缺失较免疫组织化学方法检测p16蛋白的敏感度及特异度均高,其可能在鉴别恶性间皮瘤与良性间皮增生中发挥重要的作用。
目的:探討p16基因缺失及p16蛋白的錶達情況在鑒彆噁性間皮瘤與良性反應性間皮細胞增生中的作用。方法採用熒光原位雜交( FISH)和免疫組織化學分彆檢測55例間皮瘤及30例良性反應性間皮細胞增生中p16基因和蛋白的錶達情況,對兩種方法的檢測結果進行對比分析。結果在55例噁性間皮瘤組織學標本中,p16基因缺失率為81.8%(45/55),高于良性間皮細胞增生者(3.3%,1/30),差異有統計學意義( P <0.05);噁性間皮瘤組織學標本中 p16蛋白錶達率為23.6%,低于良性間皮細胞增生者(76.7%);FISH檢測噁性間皮瘤組織中p16基因缺失敏感度及特異度均高于免疫組織化學方法檢測p16蛋白者。結論噁性間皮瘤中p16基因缺失,p16蛋白陰性錶達。 FISH檢測噁性間皮瘤組織中p16基因缺失較免疫組織化學方法檢測p16蛋白的敏感度及特異度均高,其可能在鑒彆噁性間皮瘤與良性間皮增生中髮揮重要的作用。
목적:탐토p16기인결실급p16단백적표체정황재감별악성간피류여량성반응성간피세포증생중적작용。방법채용형광원위잡교( FISH)화면역조직화학분별검측55례간피류급30례량성반응성간피세포증생중p16기인화단백적표체정황,대량충방법적검측결과진행대비분석。결과재55례악성간피류조직학표본중,p16기인결실솔위81.8%(45/55),고우량성간피세포증생자(3.3%,1/30),차이유통계학의의( P <0.05);악성간피류조직학표본중 p16단백표체솔위23.6%,저우량성간피세포증생자(76.7%);FISH검측악성간피류조직중p16기인결실민감도급특이도균고우면역조직화학방법검측p16단백자。결론악성간피류중p16기인결실,p16단백음성표체。 FISH검측악성간피류조직중p16기인결실교면역조직화학방법검측p16단백적민감도급특이도균고,기가능재감별악성간피류여량성간피증생중발휘중요적작용。
Objective To study the role of p16 gene mutation status as detected by fluorescence in-situ hybridization ( FISH) and p16 protein expression as detected by immunohistochemistry in differential diagnosis of malignant mesothelioma and benign mesothelial hyperplasia.Methods p16 gene mutation status and protein expression were detected by FISH and immunohistochemistry respectively in 55 cases of pleural malignant mesothelioma and 30 cases of benign mesothelial hyperplasia.Results FISH study showed that the rate of p16 deletion in malignant mesothelioma ( 81.8%,45/55 ) was higher than that in benign mesothelial hyperplasia ( 3.3%, 1/30 ) .The difference was statistically significant ( P <0.05 ) . Immunohistochemical study showed that the rate of p16 protein expression in malignant mesothelioma (23.6%) was lower than that in benign mesothelial hyperplasia ( 76.7%).The difference was also statistically significant.The sensitivity and specificity of FISH in distinguishing between mesothelioma and reactive mesothelial hyperplasia were higher than those of immunohistochemistry.Conclusions In contrast to reactive mesothelial hyperplasia, p16 gene is deleted and p16 protein is not expressed in malignant mesothelioma.The sensitivity and specificity of FISH are higher than those of immunohistochemistry in the distinction.