中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2015年
5期
333-337
,共5页
葡萄酒色痣%氨基酮戊酸%光化学疗法%细胞凋亡%模型,动物%鸡冠
葡萄酒色痣%氨基酮戊痠%光化學療法%細胞凋亡%模型,動物%鷄冠
포도주색지%안기동무산%광화학요법%세포조망%모형,동물%계관
Port-wine stain%Aminolevulinic acid%Photochemotherapy%Apoptosis%Models,animal%Chicken comb
目的:探讨外用氨基酮戊酸光动力疗法(ALA-PDT)对鲜红斑痣(PWS)动物模型鸡冠的作用和治疗鲜红斑痣的可行性。方法80只莱亨鸡随机分为10组:空白对照组、单纯 ALA 组、单纯激光75 J/cm2组、100 J/cm2组、150 J/cm2、200 J/cm2组,ALA-PDT 75 J/cm2组、100 J/cm2组、150 J/cm2组、200 J/cm2组。单纯激光组仅给予630 nm 红光照射,ALA-PDT 组外用 ALA 后给予红光照射。干预14 d、28 d 分别取材,观察鸡冠形态学、组织学变化,计算毛细血管减少率和血管内皮细胞凋亡情况。结果外用 ALA-PDT 75 J/cm2、100 J/cm2、150 J/cm2和200 J/cm2组鸡冠实验区域颜色变淡,光镜下真皮毛细血管数目减少、管径变小,部分血管内皮细胞凋亡;毛细血管减少率分别为33.53%±4.89%、52.02%±2.77%、67.48%±5.58%、88.96%±2.47%;凋亡指数分别为:63.44±1.09、88.50±6.11、94.32±3.67、113.76±10.57,与其余各组分别比较,均 P <0.01;血管内皮细胞凋亡深度分别为:201.19μm ±0.33μm、266.15μm ±1.02μm、546.09μm ±2.45μm、766.37μm ±1.08μm,各组间两两比较,均 P <0.01。结论外用 ALA-PDT 对鸡冠毛细血管有明显损伤,损伤程度和深度与红光能量密度相关;诱导血管内皮细胞凋亡可能是其治疗机制之一。
目的:探討外用氨基酮戊痠光動力療法(ALA-PDT)對鮮紅斑痣(PWS)動物模型鷄冠的作用和治療鮮紅斑痣的可行性。方法80隻萊亨鷄隨機分為10組:空白對照組、單純 ALA 組、單純激光75 J/cm2組、100 J/cm2組、150 J/cm2、200 J/cm2組,ALA-PDT 75 J/cm2組、100 J/cm2組、150 J/cm2組、200 J/cm2組。單純激光組僅給予630 nm 紅光照射,ALA-PDT 組外用 ALA 後給予紅光照射。榦預14 d、28 d 分彆取材,觀察鷄冠形態學、組織學變化,計算毛細血管減少率和血管內皮細胞凋亡情況。結果外用 ALA-PDT 75 J/cm2、100 J/cm2、150 J/cm2和200 J/cm2組鷄冠實驗區域顏色變淡,光鏡下真皮毛細血管數目減少、管徑變小,部分血管內皮細胞凋亡;毛細血管減少率分彆為33.53%±4.89%、52.02%±2.77%、67.48%±5.58%、88.96%±2.47%;凋亡指數分彆為:63.44±1.09、88.50±6.11、94.32±3.67、113.76±10.57,與其餘各組分彆比較,均 P <0.01;血管內皮細胞凋亡深度分彆為:201.19μm ±0.33μm、266.15μm ±1.02μm、546.09μm ±2.45μm、766.37μm ±1.08μm,各組間兩兩比較,均 P <0.01。結論外用 ALA-PDT 對鷄冠毛細血管有明顯損傷,損傷程度和深度與紅光能量密度相關;誘導血管內皮細胞凋亡可能是其治療機製之一。
목적:탐토외용안기동무산광동력요법(ALA-PDT)대선홍반지(PWS)동물모형계관적작용화치료선홍반지적가행성。방법80지래형계수궤분위10조:공백대조조、단순 ALA 조、단순격광75 J/cm2조、100 J/cm2조、150 J/cm2、200 J/cm2조,ALA-PDT 75 J/cm2조、100 J/cm2조、150 J/cm2조、200 J/cm2조。단순격광조부급여630 nm 홍광조사,ALA-PDT 조외용 ALA 후급여홍광조사。간예14 d、28 d 분별취재,관찰계관형태학、조직학변화,계산모세혈관감소솔화혈관내피세포조망정황。결과외용 ALA-PDT 75 J/cm2、100 J/cm2、150 J/cm2화200 J/cm2조계관실험구역안색변담,광경하진피모세혈관수목감소、관경변소,부분혈관내피세포조망;모세혈관감소솔분별위33.53%±4.89%、52.02%±2.77%、67.48%±5.58%、88.96%±2.47%;조망지수분별위:63.44±1.09、88.50±6.11、94.32±3.67、113.76±10.57,여기여각조분별비교,균 P <0.01;혈관내피세포조망심도분별위:201.19μm ±0.33μm、266.15μm ±1.02μm、546.09μm ±2.45μm、766.37μm ±1.08μm,각조간량량비교,균 P <0.01。결론외용 ALA-PDT 대계관모세혈관유명현손상,손상정도화심도여홍광능량밀도상관;유도혈관내피세포조망가능시기치료궤제지일。
Objective To investigate the effects of aminolevulinic acid-based photodynamic therapy(ALA-PDT) on chicken combs, an animal model for port wine stains (PWS), and to explore the feasibility of PWS treatment with ALA-PDT. Methods A total of 80 leghorns were randomly and equally divided into 10 groups: blank control group receiving no treatment, ALA group treated with ALA alone, four single laser groups irradiated with 630-nm red laser at 75, 100, 150 and 200 J/cm2 respectively, four ALA-PDT groups pretreated with ALA followed by 630-nm red laser radiation at 75, 100, 150 and 200 J/cm2 respectively. An area sized 1 cm × 1 cm were marked at one side of combs in all these leghorns, and served as the experiment area to receive corresponding treatment, with that in the other side as the control area. Tissue specimens were obtained on the 14th and 28th days after treatment followed by the observation of morphological and histological changes, calculation of decrement rate in capillary number, and determination of apoptosis index in vascular endothelial cells (VECs) in chicken combs. Results In all the four ALA-PDT groups, the combs became lighter in color with apoptosis of some VECs as well as a decrease in capillary count and diameter in the dermis of the experiment areas. The decrement rate in capillary number was 33.53% ± 4.89%, 52.02% ± 2.77%, 67.48% ± 5.58%and 88.96% ± 2.47% respectively, and apoptosis index in VECs was 63.44 ± 1.09, 88.50 ± 6.11, 94.32 ± 3.67 and 113.76 ± 10.57 respectively, in the 75-, 100-, 150- and 200-J/cm2 ALA-PDT groups on the 14th day after treatment, and both the decrement rate and apoptosis index in each of these groups were significantly different from those in the blank control group, ALA group, single laser groups receiving red laser radiation at the corresponding dose, and the other ALA-PDT groups (all P < 0.01)separately. The apoptosis depth of VECs, defined as the vertical distance from the basal layer to the deepest level at which VEC apoptosis occurred, was 201.19 ± 0.33 μm, 266.15 ± 1.02 μm, 546.09 ± 2.45 μm and 766.37 ± 1.08 μm respectively in the 75-, 100-, 150- and 200-J/cm2 ALA-PDT groups on the 14th day, with significant differences between these four groups (all P < 0.01). Conclusions ALA-PDT can markedly damage capillaries in the animal model of port wine stains, chicken combs, with the degree and depth of capillary damage associated with red light energy density. The induction of VEC apoptosis may be an action mechanism of ALA-PDT in the treatment of PWS.