CAJ | 학술논문

利用同源克隆技术，对拟南芥再生相关基因LEC进行同源序列比对，从大豆中克隆LEC基因两个成员的全长CDS序列，得到大豆再生相关基因GmLEC1-a，GmLEC1-b及GmLEC2-a，GmLEC2-b，用生物信息学方法对大豆GmLEC1及GmLEC2基因核苷酸序列以及蛋白质结构、亲水性和疏水性及进化树等进行分析。结果表明，大豆再生相关基因GmLEC1-a编码区长度为672 bp，编码223个氨基酸，GmLEC1-b编码区长度为681 bp，编码226个氨基酸，GmLEC2-a编码区长度为2205 bp，编码734个氨基酸，GmLEC2-b编码区长度为2196 bp，编码731个氨基酸，GmLEC蛋白为亲水性不稳定蛋白；通过对LEC蛋白功能结构域进行分析发现，GmLEC1为H4超家族成员，GmLEC2为B3超家族成员，并均与拟南芥属植物亲缘较近。
이용동원극륭기술，대의남개재생상관기인LEC진행동원서렬비대，종대두중극륭LEC기인량개성원적전장CDS서렬，득도대두재생상관기인GmLEC1-a，GmLEC1-b급GmLEC2-a，GmLEC2-b，용생물신식학방법대대두GmLEC1급GmLEC2기인핵감산서렬이급단백질결구、친수성화소수성급진화수등진행분석。결과표명，대두재생상관기인GmLEC1-a편마구장도위672 bp，편마223개안기산，GmLEC1-b편마구장도위681 bp，편마226개안기산，GmLEC2-a편마구장도위2205 bp，편마734개안기산，GmLEC2-b편마구장도위2196 bp，편마731개안기산，GmLEC단백위친수성불은정단백；통과대LEC단백공능결구역진행분석발현，GmLEC1위H4초가족성원，GmLEC2위B3초가족성원，병균여의남개속식물친연교근。
This article compared homologous sequences of regeneration-related gene LEC in Arabidopsis by using homology cloning technology, cloned the full-length CDS sequences of two members of LEC gene from soybean, got the soybean regeneration-related genes GmLEC1-a, GmLEC1-b, GmLEC2-a and GmLEC2-b. And nucleotide sequence, deduced amino acid sequence, protein structure, hydrophilic, hydrophobic and evolutionary trees of soybean GmLEC1 and GmLEC2 gene were analyzed by bioinformatics methods. The results showed that the coding region of soybean regeneration-related gene GmLEC1-a had the length of 672 bp, encoded 223 amino acids, that of GmLEC1-b had the length of 681 bp, encodes 226 amino acids, that of GmLEC2-a had the length of 2 205 bp, encoded 734 amino acids, and that of GmLEC2-b had the length of 2 196 bp, encoded 731 amino acids. GmLEC protein was hydrophilic unstable protein. Through the analysis of the functional domains of the LEC protein, we found that GmLEC1 was member of H4 superfamily, GmLEC2 was member of B3 superfamily, and both were close to Arabidopsis plants in phylogenetic.