CAJ | 학술논문

综合评价低温胁迫对葡萄砧木及栽培品种枝条抗寒性的影响，以1种葡萄砧木及3个葡萄栽培品种为试验材料，测定不同低温处理枝条相对电导率、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性，以及丙二醛（MDA）、可溶性糖、可溶性蛋白、游离脯氨酸含量，分析生理指标变化趋势。结果表明，葡萄枝条相对电导率随温度降低均呈现递增趋势，应用Logistic方程建立回归模型，确定半致死温度-20.7~-36.8℃；SOD和POD活性均先上升后下降，MDA、可溶性糖、游离脯氨酸、可溶性蛋白含量均呈现上升趋势；根据隶属函数法综合评判得出供试材料抗寒性强弱依次为贝达>无核白>红地球>力扎马特，与葡萄枝条抗寒性关系密切的生理指标为相对电导率、可溶性糖和MDA。试验可为葡萄丰产优质抗寒栽培、种质筛选和新品种培育提供理论依据。
종합평개저온협박대포도침목급재배품충지조항한성적영향，이1충포도침목급3개포도재배품충위시험재료，측정불동저온처리지조상대전도솔、초양화물기화매（SOD）화과양화물매（POD）활성，이급병이철（MDA）、가용성당、가용성단백、유리포안산함량，분석생리지표변화추세。결과표명，포도지조상대전도솔수온도강저균정현체증추세，응용Logistic방정건립회귀모형，학정반치사온도-20.7~-36.8℃；SOD화POD활성균선상승후하강，MDA、가용성당、유리포안산、가용성단백함량균정현상승추세；근거대속함수법종합평판득출공시재료항한성강약의차위패체>무핵백>홍지구>력찰마특，여포도지조항한성관계밀절적생리지표위상대전도솔、가용성당화MDA。시험가위포도봉산우질항한재배、충질사선화신품충배육제공이론의거。
The branches of one grape rootstock and three grape varieties were used as experimental materials, relative electrical conductivity, activities of SOD and POD, and contents of MDA, soluble sugar, soluble protein and free proline were investigated on different low temperature conditions. Results showed that relative electrical conductivity increased with the lowering of the temperature, regression model was established by using Logistic equation, and it was determined that lethal dose-50 temperature was from-20.7 to-36.8 ℃. Activities of SOD and POD increased first and then declined, while contents of MDA, soluble sugar, soluble protein and free proline increased with the lowering of the temperature. By the comprehensive evaluation with subordinate function, the order of varieties regarding the tolerance to the cold was Beta>Thompson Seedless>Red Globe>Rizamat. The physiological indices with close relation to the cold resistance of grape branches were relative electrical conductivity, soluble sugar and MDA. The results would provide a theoretical foundation to some extent for the cultivation of good harvest, high quality and cold resistance, germ plasm filter, breeding of new variety in grape.