东北农业大学学报
東北農業大學學報
동북농업대학학보
JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY
2015年
4期
89-93
,共5页
复方中药%山女鳟%受精卵%水霉菌
複方中藥%山女鱒%受精卵%水黴菌
복방중약%산녀준%수정란%수매균
Chinese herbal compounds%Oncorhynchus masou masou%fertilized eggs%Sapro-legniasis
文章对5种自组复方中药的2种提取方法2种消毒浓度,对山女鳟受精卵孵化期间水霉病防治效果进行筛选研究。采用水煎法及水煎+醇提混合法对复方中药有效成分进行提取,并设置正常剂量和稀释消毒组对5种复方中药的消毒效果进行比较研究。在10℃流水养殖条件下,将浓缩的药液加入吊瓶中,逐滴加入流水混匀,平列槽中试验鱼卵药液浸泡约40 min。结果表明,5种复方中药中,复方A水煎剂正常消毒剂量组能显著提高受精卵的发眼率,降低水霉菌感染造成的死亡,是适用于山女鳟受精卵孵化期间防治水霉感染的中药制剂。
文章對5種自組複方中藥的2種提取方法2種消毒濃度,對山女鱒受精卵孵化期間水黴病防治效果進行篩選研究。採用水煎法及水煎+醇提混閤法對複方中藥有效成分進行提取,併設置正常劑量和稀釋消毒組對5種複方中藥的消毒效果進行比較研究。在10℃流水養殖條件下,將濃縮的藥液加入弔瓶中,逐滴加入流水混勻,平列槽中試驗魚卵藥液浸泡約40 min。結果錶明,5種複方中藥中,複方A水煎劑正常消毒劑量組能顯著提高受精卵的髮眼率,降低水黴菌感染造成的死亡,是適用于山女鱒受精卵孵化期間防治水黴感染的中藥製劑。
문장대5충자조복방중약적2충제취방법2충소독농도,대산녀준수정란부화기간수매병방치효과진행사선연구。채용수전법급수전+순제혼합법대복방중약유효성분진행제취,병설치정상제량화희석소독조대5충복방중약적소독효과진행비교연구。재10℃류수양식조건하,장농축적약액가입조병중,축적가입류수혼균,평렬조중시험어란약액침포약40 min。결과표명,5충복방중약중,복방A수전제정상소독제량조능현저제고수정란적발안솔,강저수매균감염조성적사망,시괄용우산녀준수정란부화기간방치수매감염적중약제제。
Effects of five self-made Chinese medical herbs on preventing and control Saproleginiasis of Oncorhynchus masou masou fertilized eggs were evaluated in this study. The active ingredients of Chinese medical herbs here were extracted by two different methods including water decoction and combination of water decoction and ethanol extraction. The normal dosage groups and dilution groups were set up to compare the effects of five different Chinese herbal compounds with two disinfection concentrations. Under the flowing water fish culture mode at 10 ℃, concentrated drugs were added into medicine flasks and dropped into the horizontal tanks to immerse the fertilized eggs for 40 min. The results indicated that among of five kinds of Chinese herbal compounds, Compound A could improve significantly eyed rate of fertilized eggs and decrease their mortalities caused by Saprolegnia infection under the normal dosage, which could be used as a good Chinese herbs preparation to prevent and control Saprolegniasis during the hatching period.