东北农业大学学报
東北農業大學學報
동북농업대학학보
JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY
2015年
4期
83-88
,共6页
李雅娟%隋燚%赵睿%李霞%周贺%刘博%马海艳
李雅娟%隋燚%趙睿%李霞%週賀%劉博%馬海豔
리아연%수일%조예%리하%주하%류박%마해염
泥鳅%二倍体%四倍体%细胞系%染色体组%Ag-NORs%CMA3/DA/DAPI
泥鰍%二倍體%四倍體%細胞繫%染色體組%Ag-NORs%CMA3/DA/DAPI
니추%이배체%사배체%세포계%염색체조%Ag-NORs%CMA3/DA/DAPI
Misgurnus anguillicaudatus%diploid%tetraploid%cell line%genome%Ag-NORs%CMA3/DA/DAPI
对传代20次的天然二倍体和天然四倍体泥鳅鳍细胞系进行染色体标本制备,并对其染色体核型、Ag-NORs及CMA3/DA/DAPI三重荧光染色等进行研究。结果显示,①二倍体泥鳅鳍组织培养细胞的染色体数目为2n=50,核型公式为:2n=10m+4sm+36t,NF=64;四倍体泥鳅鳍组织培养细胞的染色体数目为4n=100,核型公式为:4n=20m+8sm+72t,NF=128。②天然二倍体泥鳅鳍培养的细胞中期染色体分裂相有2个Ag-NORs,天然四倍体泥鳅鳍培养的细胞中期染色体分裂相有4个Ag-NORs,均位于第1对中部着丝粒染色体(M1)的末端。③天然二倍体泥鳅鳍培养的细胞染色体中期分裂相中CMA3阳性位点为2个,天然四倍体泥鳅鳍培养的细胞染色体中期分裂相中CMA3阳性位点为4个;均位于核仁组织区,即第1对中部着丝粒染色体(M1)的末端。结果表明,天然二倍体和四倍体泥鳅鳍培养细胞制备的染色体数目、核型和带型与体细胞一致。
對傳代20次的天然二倍體和天然四倍體泥鰍鰭細胞繫進行染色體標本製備,併對其染色體覈型、Ag-NORs及CMA3/DA/DAPI三重熒光染色等進行研究。結果顯示,①二倍體泥鰍鰭組織培養細胞的染色體數目為2n=50,覈型公式為:2n=10m+4sm+36t,NF=64;四倍體泥鰍鰭組織培養細胞的染色體數目為4n=100,覈型公式為:4n=20m+8sm+72t,NF=128。②天然二倍體泥鰍鰭培養的細胞中期染色體分裂相有2箇Ag-NORs,天然四倍體泥鰍鰭培養的細胞中期染色體分裂相有4箇Ag-NORs,均位于第1對中部著絲粒染色體(M1)的末耑。③天然二倍體泥鰍鰭培養的細胞染色體中期分裂相中CMA3暘性位點為2箇,天然四倍體泥鰍鰭培養的細胞染色體中期分裂相中CMA3暘性位點為4箇;均位于覈仁組織區,即第1對中部著絲粒染色體(M1)的末耑。結果錶明,天然二倍體和四倍體泥鰍鰭培養細胞製備的染色體數目、覈型和帶型與體細胞一緻。
대전대20차적천연이배체화천연사배체니추기세포계진행염색체표본제비,병대기염색체핵형、Ag-NORs급CMA3/DA/DAPI삼중형광염색등진행연구。결과현시,①이배체니추기조직배양세포적염색체수목위2n=50,핵형공식위:2n=10m+4sm+36t,NF=64;사배체니추기조직배양세포적염색체수목위4n=100,핵형공식위:4n=20m+8sm+72t,NF=128。②천연이배체니추기배양적세포중기염색체분렬상유2개Ag-NORs,천연사배체니추기배양적세포중기염색체분렬상유4개Ag-NORs,균위우제1대중부착사립염색체(M1)적말단。③천연이배체니추기배양적세포염색체중기분렬상중CMA3양성위점위2개,천연사배체니추기배양적세포염색체중기분렬상중CMA3양성위점위4개;균위우핵인조직구,즉제1대중부착사립염색체(M1)적말단。결과표명,천연이배체화사배체니추기배양세포제비적염색체수목、핵형화대형여체세포일치。
Fin cel s of natural diploid and tetraploid loaches subcultured after 20 generations were used for chromosome preparation, and karyotype, Ag-NORs, and CMA3/DA/DAPI were studied primarily. Results showed as follows:①Chromosome number of diploid cells was 2n=50, karyotype was 2n=10m+4sm+36t, NF=64;and chromosome number of tetraploid was 4n=100, karyotype was 4n=20m+8sm+72t. NF=128.②There were 2 Ag-NORs in the medium chromosome spread of diploid, and 4 in tetraploid, all in the terminal of the first metacentric chromosome(M1).③Positive CMA3 sites in diploid were 2, and 4 in tetraploid, all in the neuclear organizer region, as the terminal of the first metacentric chromosome(M1). In conclusion, chromosome number, karyotype and band type of cultured cells of both diploid and tetraploid were consistent with somatic cells. Therefore, methods in this study could be of great value for researches of cytogenetic and molecular genetics of loaches.