CAJ | 학술논문

目的应用慢病毒介导的RNA干扰技术,对该技术能特异性的抑制黑色素瘤细胞转移进行研究.方法以人巢蛋白(Human nestin)的信使RNA编码序列作为干扰靶点,把慢病毒基因PLL3.7作为质粒载体,人巢蛋白的“短发夹”RNA(shRNA)表达质粒和阴性对照shRNA表达质粒(不包含所有已知信使RNA),分别感染黑色素瘤细胞株UACC903细胞并流式分选获得高纯度的巢蛋白干扰或对照组细胞.用划痕法检测评估各组黑色素瘤细胞迁移能力.结果巢蛋白下调的UACC903细胞侵袭能力和迁移能力均下降(P＜0.05).结论慢病毒介导的RNA能有效地沉默巢蛋白基因的表达,从而能特异性的抑制黑色素瘤细胞迁移.
목적 응용만병독개도적RNA간우기술,대해기술능특이성적억제흑색소류세포전이진행연구.방법 이인소단백(Human nestin)적신사RNA편마서렬작위간우파점,파만병독기인PLL3.7작위질립재체,인소단백적“단발협”RNA(shRNA)표체질립화음성대조shRNA표체질립(불포함소유이지신사RNA),분별감염흑색소류세포주UACC903세포병류식분선획득고순도적소단백간우혹대조조세포.용화흔법검측평고각조흑색소류세포천이능력.결과 소단백하조적UACC903세포침습능력화천이능력균하강(P＜0.05).결론 만병독개도적RNA능유효지침묵소단백기인적표체,종이능특이성적억제흑색소류세포천이.
Objective To study on the application of on the specifically inhibition the transfering.Methods Human nestin messenger RNA encoding sequence acted as the interference target.The lentiviral gene PLL3.7 acted as a plasmid vector,and used to infect melanoma cell line UACC903.Scratch melanoma cell assay was used to assess the migration ability in melanoma cell.Results UACC903 cells invasion and migration ability of nestin down-regulation were decreased (P ＜ 0.05).Conclusions RNA interference with lentiviral mediated could effectively silence the expression of nestin gene,which could specific inhibit melanoma cell migration to a certain extent.