CAJ | 학술논문

目的了解2011-2014年青岛地区人群甲型H3N2流感病毒流行株基因进化及抗原变异趋势.方法选取2011-2014年间青岛地区流行的甲型H3N2流感病毒64株,提取病毒RNA,应用逆转录聚合酶链反应(RT-PCR)扩增HA、NA、MP 3个基因片段,并进行序列测定,对各基因片段进行系统发育分析及基因和氨基酸位点变异分析.结果 HA进化树分析表明,甲型H3N2流感病毒基本上分为三大分支,并且每个分支与当年的疫苗株都不在同一分支上;HA1蛋白抗原决定簇共有8个位点发生了变化;NA蛋白酶活性中心及周围相关位点氨基酸组成保守,未检测到耐奥司他韦和扎那米韦的变异位点.M2蛋白均发生S31N突变.结论 2011-2014年青岛地区流行的H3N2流感病毒在持续不断地发生基因变异而产生抗原漂移;毒株全部为烷胺类药物耐药株,但对神经氨酸酶抑制剂敏感.
목적 료해2011-2014년청도지구인군갑형H3N2류감병독류행주기인진화급항원변이추세.방법 선취2011-2014년간청도지구류행적갑형H3N2류감병독64주,제취병독RNA,응용역전록취합매련반응(RT-PCR)확증HA、NA、MP 3개기인편단,병진행서렬측정,대각기인편단진행계통발육분석급기인화안기산위점변이분석.결과 HA진화수분석표명,갑형H3N2류감병독기본상분위삼대분지,병차매개분지여당년적역묘주도불재동일분지상;HA1단백항원결정족공유8개위점발생료변화;NA단백매활성중심급주위상관위점안기산조성보수,미검측도내오사타위화찰나미위적변이위점.M2단백균발생S31N돌변.결론 2011-2014년청도지구류행적H3N2류감병독재지속불단지발생기인변이이산생항원표이;독주전부위완알류약물내약주,단대신경안산매억제제민감.
Objective To study the prevalence and variation of influenza A (H3N2) viruses circulating in Qingdao from 2011 to 2014.Methods RNA of 64 influenza A/H3N2 virus isolates circulated in Qingdao from 2011 to 2014 was extracted.HA,NA and MP segments were amplified and sequenced.Each sequence data was analyzed using Sequencher,phylogenetic analysis using the MEGA5.0.Results HA1 gene phylogenetic tree analysis showed that there was three branches in 2011-2012,2012-2013,2013-2014 respectively,and each branch had nohomology with the vaccine strains of the same year.Compared with the vaccine strains,eight antigen sites were substituted in HA1 area;Residues at the enzyme active sites of NA protein were strictly conservative,and no change was observed in amino acid residues which were related to drug resistance against osehamivir and zanamivir.All the isolations had a S31N mutation in M2 protein.Conclusions All the A H3N2 influenza virus circulated in Qingdao from 2011 to 2014 presented continual variation and therefore caused antigenic drift.All the isolations were adamantane-resistance,but susceptible to inhibitors of neruaminidase.