CAJ | 학술논문

目的本实验通过比较重组溶瘤腺病毒RCAdC68-EGFP与复制缺陷型重组腺病毒AdC68-EGFP对结肠癌细胞的杀伤作用,初步观察RCAdC68-EGFP对结肠癌细胞增殖的影响.方法以不同病毒剂量(100、500、2500、12500vp/cell)感染结肠癌细胞HCT 116、NCI-H508、HT-29及人胚肾细胞HEK-293.感染后第3、5、7天用MTT法检测不同细胞的抑制率,结晶紫染色实验检测细胞的杀伤作用及相差显微镜观察细胞形态学变化和病变情况.结果 RCAdC68-EGFP对结肠癌细胞HCT 116、NCI-H508、HT-29的增殖抑制作用与病毒感染剂量及感染时间成正比,以500vp/cell病毒感染剂量感染细胞7天后,RCAdC68-EGFP对HCT 116、NCI-H508、HT-29、HEK293细胞的抑制率分别为86.70％、96.85％、99.70％、99.99％,AdC68-EGFP的细胞抑制率分别为6.57％、11.46％、9.11％、99.99％,RCAdC68-EGFP与AdC68-EGFP对细胞的增殖抑制作用有统计学差异(P＜0.01),而对HEK-293细胞增殖抑制作用无统计学差异(P＞0.05).结论 RCAdC68-EGFP能显著抑制HCT 116、NCI-H508、HT-29细胞的增殖,初步显示出RCAdC68-EGFP作为溶瘤病毒载体治疗肿瘤的潜能.
목적 본실험통과비교중조용류선병독RCAdC68-EGFP여복제결함형중조선병독AdC68-EGFP대결장암세포적살상작용,초보관찰RCAdC68-EGFP대결장암세포증식적영향.방법 이불동병독제량(100、500、2500、12500vp/cell)감염결장암세포HCT 116、NCI-H508、HT-29급인배신세포HEK-293.감염후제3、5、7천용MTT법검측불동세포적억제솔,결정자염색실험검측세포적살상작용급상차현미경관찰세포형태학변화화병변정황.결과 RCAdC68-EGFP대결장암세포HCT 116、NCI-H508、HT-29적증식억제작용여병독감염제량급감염시간성정비,이500vp/cell병독감염제량감염세포7천후,RCAdC68-EGFP대HCT 116、NCI-H508、HT-29、HEK293세포적억제솔분별위86.70％、96.85％、99.70％、99.99％,AdC68-EGFP적세포억제솔분별위6.57％、11.46％、9.11％、99.99％,RCAdC68-EGFP여AdC68-EGFP대세포적증식억제작용유통계학차이(P＜0.01),이대HEK-293세포증식억제작용무통계학차이(P＞0.05).결론 RCAdC68-EGFP능현저억제HCT 116、NCI-H508、HT-29세포적증식,초보현시출RCAdC68-EGFP작위용류병독재체치료종류적잠능.
Objective To explore the effect of RCAdC68-EGFP on colon carcinoma cells proliferation by comparing the killing activity of RCAdC68-EGFP and AdC68-EGFP on colon carcinoma cells.Methods Different doses of viruses (100、500、2500、12500vp/cell) were used to infect colon carcinoma cells HCT 116,NCI-HS08,HT-29 and human embryonic kidney cell HEK-293.MTT assay was used to detect the inhibition rates of viruses on different cell lines.Crystal violet staining assay was used to detect cell killing activity and phase contrast light microscopy was used to observe cell morphology and cytopathic effect (CPE) at 大 day 3,5,7 after infection.Results The killing activities of RCAdC68-EGFP on colon carcinoma cells HCT 116,NCI-H508,HT-29 were proportional to the dose and duration of virus infection.RCAdC68-EGFP inhibition rates on HCT 116、NCI-H508、HT-29 、HEK293 were 86.70、96.85％、99.70％、99.99％ respectively at the 7th day with the dose of 500vp/cell.AdC68-EGFP inhibition rates on these cells were 6.57％、11.46％、9.11％、99.99％ and 99.99％ respectively.Significant differences were found between the two adenovirus groups (P ＜ 0.05).No significant difference of inhibition was found in HEK-293 (P ＞ 0.05).Conclusions RCAdC68-GFP inhibits the proliferation of HCT116,NCI-H508 and HT-29.This finding provides supporting evidence for the future usage of RCAdC68-EGFP in the treatment of colon carcinoma.