CAJ | 학술논문

目的确定北京市急性出血性结膜炎的病原学及流行病学特征.方法 2010-2013年7月至10月在北京地区所有区县监测点收集急性结膜炎患者眼拭子标本,通过RT-PCR分别鉴定柯萨奇病毒A24变种(CVA24v)和肠道病毒70(EV70).分析急性出血性结膜炎的时间分布、患者性别、年龄及临床症状特征,利用系统发育树分析CA24v的VP1区、3Cpro区基因特征.结果随机抽取的1144件急性结膜炎样本中,4.5％(52/1144)为CVA24v阳性,未检测到EV70.CVA24v相关急性出血性结膜炎在9月达到高峰.各年龄组发病差异有统计学意义,10-19岁年龄组发病率最高.CA24v和非CA24v急性结膜炎的症状构成比无统计学差异.北京市2007-2012年分离的CA24v株VP1、3Cpro的核苷酸序列同源性分别为95.2％-100％和92.8％-100％.结论 2010-2013年CVA24v是引起北京市急性出血性结膜炎的最主要病原体.
목적 학정북경시급성출혈성결막염적병원학급류행병학특정.방법 2010-2013년7월지10월재북경지구소유구현감측점수집급성결막염환자안식자표본,통과RT-PCR분별감정가살기병독A24변충(CVA24v)화장도병독70(EV70).분석급성출혈성결막염적시간분포、환자성별、년령급림상증상특정,이용계통발육수분석CA24v적VP1구、3Cpro구기인특정.결과 수궤추취적1144건급성결막염양본중,4.5％(52/1144)위CVA24v양성,미검측도EV70.CVA24v상관급성출혈성결막염재9월체도고봉.각년령조발병차이유통계학의의,10-19세년령조발병솔최고.CA24v화비CA24v급성결막염적증상구성비무통계학차이.북경시2007-2012년분리적CA24v주VP1、3Cpro적핵감산서렬동원성분별위95.2％-100％화92.8％-100％.결론 2010-2013년CVA24v시인기북경시급성출혈성결막염적최주요병원체.
Objective To determine the etiology characteristics of acute hemorrhagic conjunctivitis in Beijing.Methods Surveillance of acute conjunctivitis was implemented during July-October in 2010-2013 by collecting eye swabs from patients consulting to surveillance hospitals affiliated to 18 districts Center for Disease Prevention and Control (CDC) in Beijing.Coxsackievirus A24 variant (CA24v),Enterovirus 70 (EV70) were identified by RT-PCR.Time distribution and characteristics about the cases were described.Phylogenetic analysis of VP1 and 3Cpro of CA24v were conducted.Comparison of proportions and statistical significance were calculated using the Chi-square test.Results About 4.5％ (52/1144) cases were CVA24v infected cases.EV70 didn't be identified.There was no significant difference between CVA24v and non-CVA24v in term of six symptoms.Prevalence peak of CVA24v mediated conjunctivitis was observed at September.Nucleotide sequence of VP1,3Cpro among isolated CVA24v strains had a range of 95.2％-100％ and 92.8％-100％ identity value,respectively.Conclusions CVA24v was the most major agent to cause acute conjunctivitis in Beijing.