目的：观察先天性白内障小鼠发育过程中视网膜Acin1基因( apoptotic chromatin condensation inducer 1,凋亡染色体凝聚诱导1)表达的变化及差异,探讨先天性白内障小鼠与正常小鼠视网膜细胞凋亡的差异及晶状体与视网膜发育的联系。<br> 方法：选取先天性白内障小鼠和正常C57 BL/6小鼠各15只,其中雌性各10只,雄性各5只,两种小鼠各随机按1雄2雌合笼正常饲养,雌鼠受孕后单独饲养,待其分娩后将对应幼鼠分为先天性白内障组和正常对照组,各组于1,5,9,14,17,21,26,60d 分别处理5只幼鼠,取左眼球4%中性甲醛固定行免疫组化检测ACIN1蛋白( ACINUS )表达,右眼取视网膜行PCR检测Acin1 mRNA表达。<br> 结果：Acin1在两组幼鼠视网膜各日龄均有持续表达；随着视网膜各层细胞的分化,ACIN1蛋白从神经节细胞层、内核层至外核层逐渐表达,以神经节细胞和内核层为主,神经母细胞层未见阳性表达,正常对照组P1~P14 d ACIN1阳性细胞逐渐增多, P17 d开始减少, P21 d之后各层阳性细胞明显减少；先天性白内障组整体变化趋势同正常对照组类似, P1~P14 d 阳性细胞数低于正常对照组, P17~P21 d阳性细胞数持平且高于正常对照组；同日龄两组比较,除P17, P26, P60 d外,其余差异均有统计学意义( P<0.05)；先天性白内障组内不同日龄总体比较差异有统计学意义(F先白=295.07,P<0.01),两两比较除P1与P5d, P17与P21 d外其余各日龄间均有统计学意义( P<0.05)；正常组内不同日龄总体比较差异有统计学意义(F正常=214.21,P<0.01),除P1与P5d外其余各日龄间两两比较均有统计学意义(P<0.05)；先天性白内障组P17d表达量低于正常组 P14 d 差异有统计学意义( P<0.05)。两组Acin1 mRNA变化趋势与蛋白相似,两组同日龄比较,除P17,P21,P60d外差异均有统计学意义(P<0.05)；两组组内不同日龄总体比较差异均有统计学意义(F先白=522.29,P<0.01；F正常=472.05,P<0.01)；两两比较先天性白内障组除P21与P26 d外其余各日龄间均有统计学差异(P<0.05),正常组各日龄间两两比较均有统计学差异(P<0.05)。<br> 结论：小鼠视网膜发育过程中存在Acin1时间和空间的差异性表达,先天性白内障晶状体发育障碍可能会影响视网膜细胞Acin1的表达及视网膜细胞的凋亡及发育。
目的：觀察先天性白內障小鼠髮育過程中視網膜Acin1基因( apoptotic chromatin condensation inducer 1,凋亡染色體凝聚誘導1)錶達的變化及差異,探討先天性白內障小鼠與正常小鼠視網膜細胞凋亡的差異及晶狀體與視網膜髮育的聯繫。<br> 方法：選取先天性白內障小鼠和正常C57 BL/6小鼠各15隻,其中雌性各10隻,雄性各5隻,兩種小鼠各隨機按1雄2雌閤籠正常飼養,雌鼠受孕後單獨飼養,待其分娩後將對應幼鼠分為先天性白內障組和正常對照組,各組于1,5,9,14,17,21,26,60d 分彆處理5隻幼鼠,取左眼毬4%中性甲醛固定行免疫組化檢測ACIN1蛋白( ACINUS )錶達,右眼取視網膜行PCR檢測Acin1 mRNA錶達。<br> 結果：Acin1在兩組幼鼠視網膜各日齡均有持續錶達；隨著視網膜各層細胞的分化,ACIN1蛋白從神經節細胞層、內覈層至外覈層逐漸錶達,以神經節細胞和內覈層為主,神經母細胞層未見暘性錶達,正常對照組P1~P14 d ACIN1暘性細胞逐漸增多, P17 d開始減少, P21 d之後各層暘性細胞明顯減少；先天性白內障組整體變化趨勢同正常對照組類似, P1~P14 d 暘性細胞數低于正常對照組, P17~P21 d暘性細胞數持平且高于正常對照組；同日齡兩組比較,除P17, P26, P60 d外,其餘差異均有統計學意義( P<0.05)；先天性白內障組內不同日齡總體比較差異有統計學意義(F先白=295.07,P<0.01),兩兩比較除P1與P5d, P17與P21 d外其餘各日齡間均有統計學意義( P<0.05)；正常組內不同日齡總體比較差異有統計學意義(F正常=214.21,P<0.01),除P1與P5d外其餘各日齡間兩兩比較均有統計學意義(P<0.05)；先天性白內障組P17d錶達量低于正常組 P14 d 差異有統計學意義( P<0.05)。兩組Acin1 mRNA變化趨勢與蛋白相似,兩組同日齡比較,除P17,P21,P60d外差異均有統計學意義(P<0.05)；兩組組內不同日齡總體比較差異均有統計學意義(F先白=522.29,P<0.01；F正常=472.05,P<0.01)；兩兩比較先天性白內障組除P21與P26 d外其餘各日齡間均有統計學差異(P<0.05),正常組各日齡間兩兩比較均有統計學差異(P<0.05)。<br> 結論：小鼠視網膜髮育過程中存在Acin1時間和空間的差異性錶達,先天性白內障晶狀體髮育障礙可能會影響視網膜細胞Acin1的錶達及視網膜細胞的凋亡及髮育。
목적：관찰선천성백내장소서발육과정중시망막Acin1기인( apoptotic chromatin condensation inducer 1,조망염색체응취유도1)표체적변화급차이,탐토선천성백내장소서여정상소서시망막세포조망적차이급정상체여시망막발육적련계。<br> 방법：선취선천성백내장소서화정상C57 BL/6소서각15지,기중자성각10지,웅성각5지,량충소서각수궤안1웅2자합롱정상사양,자서수잉후단독사양,대기분면후장대응유서분위선천성백내장조화정상대조조,각조우1,5,9,14,17,21,26,60d 분별처리5지유서,취좌안구4%중성갑철고정행면역조화검측ACIN1단백( ACINUS )표체,우안취시망막행PCR검측Acin1 mRNA표체。<br> 결과：Acin1재량조유서시망막각일령균유지속표체；수착시망막각층세포적분화,ACIN1단백종신경절세포층、내핵층지외핵층축점표체,이신경절세포화내핵층위주,신경모세포층미견양성표체,정상대조조P1~P14 d ACIN1양성세포축점증다, P17 d개시감소, P21 d지후각층양성세포명현감소；선천성백내장조정체변화추세동정상대조조유사, P1~P14 d 양성세포수저우정상대조조, P17~P21 d양성세포수지평차고우정상대조조；동일령량조비교,제P17, P26, P60 d외,기여차이균유통계학의의( P<0.05)；선천성백내장조내불동일령총체비교차이유통계학의의(F선백=295.07,P<0.01),량량비교제P1여P5d, P17여P21 d외기여각일령간균유통계학의의( P<0.05)；정상조내불동일령총체비교차이유통계학의의(F정상=214.21,P<0.01),제P1여P5d외기여각일령간량량비교균유통계학의의(P<0.05)；선천성백내장조P17d표체량저우정상조 P14 d 차이유통계학의의( P<0.05)。량조Acin1 mRNA변화추세여단백상사,량조동일령비교,제P17,P21,P60d외차이균유통계학의의(P<0.05)；량조조내불동일령총체비교차이균유통계학의의(F선백=522.29,P<0.01；F정상=472.05,P<0.01)；량량비교선천성백내장조제P21여P26 d외기여각일령간균유통계학차이(P<0.05),정상조각일령간량량비교균유통계학차이(P<0.05)。<br> 결론：소서시망막발육과정중존재Acin1시간화공간적차이성표체,선천성백내장정상체발육장애가능회영향시망막세포Acin1적표체급시망막세포적조망급발육。
?AlM: To observe the expression of Acin1 ( apoptotic chromatin condensation inducer 1 ) in congenital cataract mouse retina during development and investigate the differences of retinal apoptosis and the connection of lens and retina development between congenital cataract mouse and normal mouse. <br> ?METHODS: There were congenital cataract mice ( 10 female and 5 male) and normal C57BL/6 mice (10 female and 5 male) . One male and two female mice were fed in the same cage randomly. The young mice were divided into two groups: congenital cataract group and normal control group. Five young mice were treated each group on 1, 5, 9, 14, 17, 21, 26, 60d. The left eyes were fixed with 4% neutral formalin to detect AClN1 protein by immunohistochemistry and retinas from right eyes were used to detect the mRNA expression of Acin1. <br> ?RESULTS: Acin1 had sustained expression in each group. AClN1 protein gradually expressed from the ganglion cell layer, inner nuclear layer to the outer nuclear layer following retinal development. lt mainly expressed on ganglion cell layer and inner nuclear layer, but not neuroblastoma layer. AClN1 protein positive cells on P1 ~ P14d increased in normal control group, P17d reduced, after P21d positive cells of each layers decreased. The overall trend was similar in congenital cataract group with normal control group, P1 ~ P14d positive cells count was lower than normal control group, P17-P21d positive cells were flat and higher than the normal control group. Compared with the same day of the two groups, the differences except for P17, P26, P60d were significant (P<0. 05). The overall difference was statistically significant in congenital cataract group ( Fcataract=295. 07, P<0. 01);in addition to P1 and P5, P17 and P21, the differences were statistically significant ( P< 0. 05 ) compared with each other in congenital cataract group. The overall difference was statistically significant in control group (Fnormal=214. 21, P<0. 01); in addition to P1 and P5d, the difference was statistically significant ( P<0. 05) compared with each other in control group. The expression of P17d in congenital cataract group was lower compared with that of P14d in control group, the difference was statistically significant (P<0. 05). Acin1 mRNA trends of two groups were similar with AClN1 protein. Compared with the same day of the two groups, the difference was significant except for P17, P21, P60d (P<0. 05 ) . The overall difference was statistically significant in each other of the two groups ( Fcataract=522. 29, P<0. 01;Fnormal=472. 05, P<0. 01). The difference was statistically significant compared with each day in control group ( P<0. 05). Compared with all the rest of days except for P21 and P26d, the difference was statistically significant in congenital cataract group (P<0. 05). <br> ?CONCLUSlON: Acin1 exist differential expression of time and space in mouse retina during development, congenital cataract crystal developmental disorder may affect the expression of Acin1 and retinal cell apoptosis and development.
