CAJ | 학술논문

目的：探讨褪黑素对过氧化氢诱导晶状体上皮细胞氧化损伤的保护作用。 　　方法：晶状体上皮细胞传代培养后,分别加入不同浓度褪黑素预处理12h后,加入100μmol/L H2 O2继续孵育24h, MTT比色法检测褪黑素对H2 O2诱导的晶状体上皮细胞活力的影响,流式细胞仪检测细胞凋亡率,比色法检测凋亡相关因子Caspase-3及Caspase-9的活性。 　　结果：MTT结果显示褪黑素对晶状体上皮细胞活性无影响,该药物可以抑制过氧化氢诱导的细胞活性的下降,流式细胞计数结果显示褪黑素可以抑制过氧化氢诱导的细胞凋亡,此外,褪黑素还可以减少过氧化氢所致晶状体上皮细胞内Caspase-3及Caspase-9的活性,并且,伴随褪黑素作用时间的延长其活性呈下降趋势。 　　结论：褪黑素可以明显抑制过氧化氢诱导的晶状体上皮细胞的凋亡,从而为寻求有效的防治白内障药物提供可靠的实验依据。
목적：탐토퇴흑소대과양화경유도정상체상피세포양화손상적보호작용。 　　방법：정상체상피세포전대배양후,분별가입불동농도퇴흑소예처리12h후,가입100μmol/L H2 O2계속부육24h, MTT비색법검측퇴흑소대H2 O2유도적정상체상피세포활력적영향,류식세포의검측세포조망솔,비색법검측조망상관인자Caspase-3급Caspase-9적활성。 　　결과：MTT결과현시퇴흑소대정상체상피세포활성무영향,해약물가이억제과양화경유도적세포활성적하강,류식세포계수결과현시퇴흑소가이억제과양화경유도적세포조망,차외,퇴흑소환가이감소과양화경소치정상체상피세포내Caspase-3급Caspase-9적활성,병차,반수퇴흑소작용시간적연장기활성정하강추세。 　　결론：퇴흑소가이명현억제과양화경유도적정상체상피세포적조망,종이위심구유효적방치백내장약물제공가고적실험의거。
?AlM: To investigate theprotective effect of melatonin against hydrogen peroxide ( H2 O2 )-induced oxidative damage to human lens epithelial cells. ?METHODS: Sub-culture human lens epithelial cells preprocessed with different concentrations of melatonin for 12h and then 100 μmol/L H2 O2 for 24h. The impact of melatonin on H2 O2-induced lens epithelial cell viability was detected by MTT assay, rate of apoptosis was detected by flow cytometry instrument and activity of apoptosis-related factors, Caspase-3 and Caspase-9, were detected by colorimetric method. ?RESULTS: MTT assay showed that melatonin had no effect on the activity of lens epithelial cells, and the drug can inhibit the decrease of H2 O2-induced cell activity, as well as flow cytometry showed that melatonin can inhibit H2 O2-induced apoptosis. ln addition, melatonin can also reduce H2 O2-induced Caspase-3 and Caspase-9 activity in lens epithelial cells, and their activity decreased with effect of melatonin along with extending time. ?CONCLUSlON: Melatonin can obviously inhibit H2 O2 -induced apoptosis of human lens epithelial cells, which provide reliable experimental basis for drug on treatment of cataract.