CAJ | 학술논문

目的：探讨 miR-181在白内障晶状体组织中的表达情况,及其对人晶状体上皮细胞凋亡的调控机制。 　　方法：利用Real time q-PCR方法,检测年龄相关性白内障患者晶状体前囊膜和人晶状体上皮细胞凋亡模型中miR-181的表达情况；利用Lipofectamine 2000瞬时转染miR-181 mimic 和 inhibitor 调节人晶状体上皮细胞中miR-181的表达,利用Real time q-PCR方法验证转染效率,利用流式细胞仪检测细胞凋亡率的变化。 　　结果：与对照组相比,年龄相关性白内障患者晶状体前囊膜组和人晶状体上皮细胞凋亡模型组,miR-181的表达显著升高；miR-181 mimic转染组,miR-181的表达显著升高,细胞凋亡率显著升高；miR-181 inhibitor转染组, miR-181的表达显著降低,细胞凋亡率显著降低,差异均有统计学意义(P<0.01)。 　　结论：miR-181在年龄相关性白内障晶状体组织中呈高表达,miR-181能够促进人晶状体上皮细胞凋亡,从而可能在年龄相关性白内障的发病过程中发挥一定作用,miR-181可能成为白内障非手术治疗的一种新途径,但具体机制有待进一步研究。
목적：탐토 miR-181재백내장정상체조직중적표체정황,급기대인정상체상피세포조망적조공궤제。 　　방법：이용Real time q-PCR방법,검측년령상관성백내장환자정상체전낭막화인정상체상피세포조망모형중miR-181적표체정황；이용Lipofectamine 2000순시전염miR-181 mimic 화 inhibitor 조절인정상체상피세포중miR-181적표체,이용Real time q-PCR방법험증전염효솔,이용류식세포의검측세포조망솔적변화。 　　결과：여대조조상비,년령상관성백내장환자정상체전낭막조화인정상체상피세포조망모형조,miR-181적표체현저승고；miR-181 mimic전염조,miR-181적표체현저승고,세포조망솔현저승고；miR-181 inhibitor전염조, miR-181적표체현저강저,세포조망솔현저강저,차이균유통계학의의(P<0.01)。 　　결론：miR-181재년령상관성백내장정상체조직중정고표체,miR-181능구촉진인정상체상피세포조망,종이가능재년령상관성백내장적발병과정중발휘일정작용,miR-181가능성위백내장비수술치료적일충신도경,단구체궤제유대진일보연구。
?AlM: To investigate the expression of miR-181 in the lens tissue of cataract and the regulating mechanism of miR-181 on apoptosis of human lens epithelial cell. ?METHODS:Real time q-PCR was used to measure the expression of miR-181 in the anterior lens capsules of age - related cataract and human lens epithelial cell apoptosis model. miR- 181 mimic and inhibitor were transfected using Lipofectamine 2 000 to regulate the expression of miR-181, and then Real time q-PCR was used to verify transfection efficiency. Flow cytometry was used to detect the change of cell apoptosis rate. ? RESULTS: Compared with control group, the expression of miR-181 was significantly higher in both the anterior lens capsules of age-related cataract and human lens epithelial cell apoptosis model; the relative expression of miR-181 in lens epithelial cells transfected with miR-181 mimic was increased, whereas decreased in cells transfected with miR-181 inhibitor;the apoptosis rate of cells transfected with miR - 181 mimic was increased, while reduced in miR-181 inhibitor group. Each result was statistically significant (P<0. 01). ?CONCLUSlON:High expression of miR-181 is detected in anterior lens capsule of age-related cataract. miR-181 might play a certain role in the pathogenesis of cataract via promoting human lens epithelial cell apoptosis. miR-181 probably becomes a new approach for the nonoperative treatment of cataract, but the concrete mechanism still needs to be further studied.