CAJ | 학술논문

目的：：观察免疫斑点法和免疫印迹法测定抗 ENA 抗体结果的差异性。方法：选取美国 GenBio 公司生产的自身抗体检测试剂盒(免疫斑点法)和广州万孚公司生产 ENA 试剂盒(免疫印迹法),同时检测433例样本中的抗 ENA 抗体,结果采用 McNemar检验,比较这两种方法的检测性能。结果：(1)成组χ2检验：免疫斑点法和免疫印迹法的检测结果有相关性(P =0.000)；(2)McNemar检验：免疫斑点法和免疫印迹法检测结果之间有差异(P =0.000)；(3)Kappa 检验：免疫斑点法和免疫印迹法检测结果的一致性有统计学意义,但一致性程度一般(Kappa=0.664,P =0.000)。结论：在临床检测抗 ENA 抗体时,对于弱阳性结果、不典型的结果最好用另一种方法来检测,再结合其它指标来综合判断,才能准确地发出检验报告。
목적：：관찰면역반점법화면역인적법측정항 ENA 항체결과적차이성。방법：선취미국 GenBio 공사생산적자신항체검측시제합(면역반점법)화엄주만부공사생산 ENA 시제합(면역인적법),동시검측433례양본중적항 ENA 항체,결과채용 McNemar검험,비교저량충방법적검측성능。결과：(1)성조χ2검험：면역반점법화면역인적법적검측결과유상관성(P =0.000)；(2)McNemar검험：면역반점법화면역인적법검측결과지간유차이(P =0.000)；(3)Kappa 검험：면역반점법화면역인적법검측결과적일치성유통계학의의,단일치성정도일반(Kappa=0.664,P =0.000)。결론：재림상검측항 ENA 항체시,대우약양성결과、불전형적결과최호용령일충방법래검측,재결합기타지표래종합판단,재능준학지발출검험보고。
Objective:To observe the difference between the anti-ENA antibody detection result obtained through immunospot assay and immunoblot assay.Methods:Test anti-ENA antibody contained 433 samples with the autoantibody test kit (immunospot assay)yielded by American GenBio company and ENA test kit (immunoblot assay)yielded by Guangzhou Wondfo Biotech Co.,Ltd,check the result with McNemar,com-pare the detection performance of the 2 methods.Results:(1)Chi-square test :The result of immunospot as-say was correlative to that of immunoblot assay(P =0.000);(2)McNemar test:The result of immunospot as-say was different from that of immunoblot assay(P =0.000);(3)Kappa test:the consistency between the re-sult of immunospot assay and immunoblot assay was statistically significant,but the consistency degree was around the average (Kappa=0.664,P =0.000).Conclusion:When testing the anti-ENA antibody clinically,in allusion to the weakly positive results and atypical results,it is better to test with some other methods and combine other indexes to make comprehensive judgments,so as to give out the exact test report.