CAJ | 학술논문

目的研究衰老对大鼠肠系膜上动脉内皮细胞(MAECs)钙库操纵的钙离子内流(SOCE)及其介导的血管舒张功能的影响.方法采用血管直径测量技术比较老年和非老年大鼠缓激肽引起肠系膜上动脉血管舒张反应;原代培养大鼠MAECs;利用毒胡萝卜素和缓激肽清空内质网上的钙库,采用钙成像技术检测老年和非老年大鼠MAECs细胞外Ca2+内流情况;通过免疫荧光染色技术比较钙离子通道(Orai 1)蛋白和基质相互作用因子(STIM 1)在老年和非老年大鼠MAECs表达情况.结果与非老年大鼠(70.0±4.1)％相比,缓激肽引起的老年大鼠(27.0±4.9)％肠系膜上动脉血管舒张功能减弱了(60.7±4.3)％;在原代培养的MAECs,毒胡萝卜素(荧光强度变化非老年和老年分别是2.72±0.39和1.71±0.48)与缓激肽(非老年和老年分别是1.75±0.06和1.06±0.03)诱导的SOCE分别减弱37.6％与39.2％;SOCE的组成成分Orai 1和STIM 1表达明显减少.结论衰老引起大鼠MAECs的SOCE及其介导的血管舒张功能显著减弱,钙池调控钙离子通道组成蛋白Orai 1与STIM1蛋白表达水平降低可能参与改变这一现象.
목적 연구쇠로대대서장계막상동맥내피세포(MAECs)개고조종적개리자내류(SOCE)급기개도적혈관서장공능적영향.방법 채용혈관직경측량기술비교노년화비노년대서완격태인기장계막상동맥혈관서장반응;원대배양대서MAECs;이용독호라복소화완격태청공내질망상적개고,채용개성상기술검측노년화비노년대서MAECs세포외Ca2+내류정황;통과면역형광염색기술비교개리자통도(Orai 1)단백화기질상호작용인자(STIM 1)재노년화비노년대서MAECs표체정황.결과 여비노년대서(70.0±4.1)％상비,완격태인기적노년대서(27.0±4.9)％장계막상동맥혈관서장공능감약료(60.7±4.3)％;재원대배양적MAECs,독호라복소(형광강도변화비노년화노년분별시2.72±0.39화1.71±0.48)여완격태(비노년화노년분별시1.75±0.06화1.06±0.03)유도적SOCE분별감약37.6％여39.2％;SOCE적조성성분Orai 1화STIM 1표체명현감소.결론 쇠로인기대서MAECs적SOCE급기개도적혈관서장공능현저감약,개지조공개리자통도조성단백Orai 1여STIM1단백표체수평강저가능삼여개변저일현상.
Objective To observe attenuated store-operated Ca2+ entry in superior mesenteric artery endothelial cells of aged rat.Methods The pressure myograph was applied to measure the vasodilation of superior mesenteric artery induced by bradykinin (BK) in aged versus non-elder rats.Primarily cultured MAECs were treated by thapsigargin (TG) and BK to deplete intracellular Ca2+ stores for comparing SOCE.SOCE of MAECs was detected by calcium imaging technology and was compared between aged and non-elder rats.The expression levels of Orai 1 and stromal interaction factor 1 (STIM 1) were observed by immunofluorescence method.Results Compared with the nonelder rats (70.0 ± 4.1 ％),BK-induced vasodilation in aged rats (27.0 ± 4.9)％ was declined by (60.7±4.3) ％.SOCE induced by BK and TG in primarily cultured MAECs was attenuated by 37.6％ and 39.2％ in aged rats (1.71±0.18 and 1.06±0.03) respectively as compared with non-elder rats (2.72±0.39 and 1.75±0.06).The expressions of SOCE's components Orai 1 and STIM 1 in MAECs were decreased obviously in aged rats than in non elder rats.Conclusions SOCE of MAECs and SOCE-induced vasodilation are significantly attenuated in aged rats.The decreased expression level of Orai 1 and STIM 1 may contribute to this alteration.